BAIT
APH1B
2310057K14Rik, Aph1c
anterior pharynx defective 1b homolog (C. elegans)
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
STXBP1
AI317162, AI326233, MMS10-G, Ms10g, Munc-18a, Munc18-1, N-sec1, Rb-sec1, Sxtbp1, Unc18-1, Unc18h, nsec1, RP23-17P12.13-002
syntaxin binding protein 1
GO Process (16)
GO Function (9)
GO Component (10)
Gene Ontology Biological Process
- axon target recognition [IMP]
- exocytosis [IMP]
- long term synaptic depression [IMP]
- negative regulation of neuron apoptotic process [IMP]
- negative regulation of synaptic transmission, GABAergic [IDA]
- neuromuscular synaptic transmission [IMP]
- neurotransmitter secretion [IMP]
- platelet aggregation [ISO]
- platelet degranulation [ISO]
- positive regulation of calcium ion-dependent exocytosis [IDA]
- positive regulation of exocytosis [ISO]
- protein localization to plasma membrane [ISO]
- protein stabilization [ISO]
- regulation of synaptic vesicle priming [ISO]
- synaptic vesicle maturation [IMP]
- vesicle docking involved in exocytosis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID