BAIT
PRNP
AA960666, AI325101, CD230, PrP, PrP, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C, RP23-401J24.1
prion protein
GO Process (19)
GO Function (7)
GO Component (10)
Gene Ontology Biological Process
- cellular copper ion homeostasis [TAS]
- cellular response to copper ion [ISO]
- cellular response to drug [IDA]
- learning or memory [ISO]
- negative regulation of T cell receptor signaling pathway [IMP]
- negative regulation of activated T cell proliferation [IMP]
- negative regulation of apoptotic process [IGI, ISO]
- negative regulation of calcineurin-NFAT signaling cascade [IMP]
- negative regulation of interferon-gamma production [IMP]
- negative regulation of interleukin-17 production [IMP]
- negative regulation of interleukin-2 production [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- nucleobase-containing compound metabolic process [TAS]
- regulation of potassium ion transmembrane transport [IGI]
- regulation of protein localization [IMP]
- response to cadmium ion [ISO]
- response to copper ion [ISO]
- response to oxidative stress [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP1B1
Atp4b, Atpb, Atpb-1, NKbeta1
ATPase, Na+/K+ transporting, beta 1 polypeptide
GO Process (26)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- blastocyst development [IMP]
- cardiac muscle contraction [IMP]
- cellular calcium ion homeostasis [IMP]
- cellular potassium ion homeostasis [ISO]
- cellular sodium ion homeostasis [ISO]
- membrane repolarization [ISO]
- metal ion transport [ISO]
- positive regulation of ATP catabolic process [ISO]
- positive regulation of ATPase activity [ISO]
- positive regulation of calcium:sodium antiporter activity [IMP]
- positive regulation of potassium ion import [ISO]
- positive regulation of potassium ion transmembrane transporter activity [ISO]
- positive regulation of sodium ion export from cell [ISO]
- potassium ion import [IC, ISO]
- potassium ion transport [ISO]
- protein localization to plasma membrane [ISO]
- protein stabilization [IMP, ISO]
- regulation of calcium ion transmembrane transport [IMP]
- regulation of cardiac muscle contraction by calcium ion signaling [IMP]
- regulation of gene expression [IMP]
- relaxation of cardiac muscle [IMP]
- response to hypoxia [ISO]
- sodium ion export from cell [IC, ISO]
- sodium ion transmembrane transport [ISO]
- sodium ion transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues.
Because of their sensitivity to solubilizing detergents, membrane protein assemblies are difficult to study. We describe a protocol that covalently conserves protein interactions through time-controlled transcardiac perfusion cross-linking (tcTPC) before disruption of tissue integrity. To validate tcTPC for identifying protein-protein interactions, we established that tcTPC allowed stringent immunoaffinity purification of the gamma-secretase complex in high salt concentrations and detergents and ... [more]
Nat. Biotechnol. Jun. 01, 2004; 22(6);724-31 [Pubmed: 15146195]
Throughput
- Low Throughput
Curated By
- BioGRID