An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The RING finger protein SNURF is a bifunctional protein possessing DNA binding activity.

Haekli M, Karvonen U, Jaenne OA, Palvimo JJ

The small nuclear C(3)HC(4) finger protein (SNURF), RNF4, acts as transcriptional coactivator for both steroid-dependent and -independent promoters such as those driven by androgen response elements and GC boxes, respectively. However, SNURF does not possess intrinsic transcription activation function, and the precise molecular mechanism of its action is unknown. We have studied herein the interaction of SNURF with DNA in ... [more]

J. Biol. Chem. Jun. 29, 2001; 276(26);23653-60 [Pubmed: 11319220]

Throughput

Low Throughput

Additional Notes

GST-Rnf4 (Snurf) was incubated with or without rat thymus histones and examined for binding

Curated By

BioGRID

Interaction Summary

RNF4

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]SUMO polymer binding [IPI]TBP-class protein binding [TAS]androgen receptor binding [IPI]estrogen receptor binding [IDA]nucleosome binding [IDA]progesterone receptor binding [IPI]protein binding [IPI]transcription factor binding [ISO]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

HIST1H2BL