An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

DJ-1 associates with synaptic membranes.

Usami Y, Hatano T, Imai S, Kubo S, Sato S, Saiki S, Fujioka Y, Ohba Y, Sato F, Funayama M, Eguchi H, Shiba K, Ariga H, Shen J, Hattori N

Parkinson's disease (PD) is a neurodegenerative disorder caused by loss of dopaminergic neurons. Although many reports have suggested that genetic factors are implicated in the pathogenesis of PD, molecular mechanisms underlying selective dopaminergic neuronal degeneration remain unknown. DJ-1 is a causative gene for autosomal recessive form of PARK7-linked early-onset PD. A number of studies have demonstrated that exogenous DJ-1 localizes ... [more]

Neurobiol. Dis. Sep. 01, 2011; 43(3);651-62 [Pubmed: 21645620]

Throughput

Low Throughput

Additional Notes

figure 4. Immunoisolation instead of immnuoprecipitation.

Curated By

BioGRID

Interaction Summary

PARK7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]peroxiredoxin activity [IMP]

Gene Ontology Cellular Component

VAMP2