BAIT
SNCA
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (70)
GO Function (26)
GO Component (25)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- adult locomotory behavior [ISO]
- aging [IEP]
- behavioral response to cocaine [IMP]
- calcium ion homeostasis [ISO]
- cellular response to copper ion [ISO]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to oxidative stress [ISO]
- dopamine biosynthetic process [ISO]
- dopamine metabolic process [ISO]
- fatty acid metabolic process [ISO]
- long-term synaptic potentiation [ISO]
- membrane organization [ISO]
- microglial cell activation [ISO]
- mitochondrial ATP synthesis coupled electron transport [ISO]
- mitochondrial membrane organization [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of dopamine metabolic process [IMP]
- negative regulation of dopamine uptake involved in synaptic transmission [ISO]
- negative regulation of exocytosis [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of microtubule polymerization [ISO]
- negative regulation of monooxygenase activity [ISO]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of norepinephrine uptake [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of serotonin uptake [ISO]
- negative regulation of thrombin receptor signaling pathway [ISO]
- negative regulation of transporter activity [ISO]
- neutral lipid metabolic process [ISO]
- oxidation-reduction process [ISO]
- phospholipid metabolic process [ISO]
- positive regulation of endocytosis [ISO]
- positive regulation of glutathione peroxidase activity [ISO]
- positive regulation of hydrogen peroxide catabolic process [ISO]
- positive regulation of inositol phosphate biosynthetic process [ISO]
- positive regulation of neurotransmitter secretion [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of receptor recycling [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission [ISO]
- protein destabilization [ISO]
- receptor internalization [ISO]
- regulation of acyl-CoA biosynthetic process [ISO]
- regulation of dopamine secretion [ISO]
- regulation of excitatory postsynaptic membrane potential [ISO]
- regulation of glutamate secretion [ISO]
- regulation of locomotion [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of macrophage activation [ISO]
- regulation of neuron apoptotic process [ISO]
- regulation of neuron death [IGI]
- regulation of neuronal synaptic plasticity [ISO]
- regulation of neurotransmitter secretion [ISO]
- regulation of phospholipase activity [ISO]
- response to cocaine [IEP]
- response to drug [ISO]
- response to interferon-gamma [ISO]
- response to interleukin-1 [ISO]
- response to iron(II) ion [ISO]
- response to lipopolysaccharide [ISO]
- response to magnesium ion [ISO]
- synapse organization [ISO]
- synaptic transmission [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic vesicle endocytosis [ISO]
- synaptic vesicle transport [ISO]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- actin cytoskeleton [ISO]
- axon [ISO]
- axon terminus [IDA]
- cell [ISO]
- cell cortex [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle membrane [IDA]
- cytoskeleton [ISO]
- cytosol [IDA, ISO]
- fibril [ISO]
- growth cone [IDA, ISO]
- inclusion body [ISO]
- intracellular membrane-bounded organelle [IDA]
- mitochondrion [IDA, ISO]
- nuclear outer membrane [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- platelet alpha granule membrane [ISO]
- ribosome [IDA]
- rough endoplasmic reticulum [IDA]
- synapse [ISO]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
Rattus norvegicus
PREY
PPP2CA
Pp2a1
protein phosphatase 2, catalytic subunit, alpha isozyme
GO Process (26)
GO Function (16)
GO Component (9)
Gene Ontology Biological Process
- cardiac ventricle development [IEP]
- cellular response to calcium ion [IEP]
- cellular response to cytokine stimulus [IEP]
- cellular response to ethanol [IEP]
- cellular response to follicle-stimulating hormone stimulus [IEP]
- cellular response to glucose stimulus [IEP, IMP]
- cellular response to insulin stimulus [IEP]
- cellular response to steroid hormone stimulus [IEP]
- heart development [IEP]
- mesoderm development [ISO]
- negative regulation of calcium ion transmembrane transporter activity [IMP]
- negative regulation of epithelial to mesenchymal transition [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription factor import into nucleus [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of protein dephosphorylation [IDA]
- positive regulation of protein phosphatase type 2A activity [IMP]
- positive regulation of protein serine/threonine kinase activity [ISO]
- protein dephosphorylation [IDA]
- protein heterotrimerization [IMP]
- regulation of protein autophosphorylation [IMP]
- regulation of protein catabolic process [IMP]
- regulation of protein phosphorylation [IMP]
- regulation of receptor activity [IMP]
Gene Ontology Molecular Function- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
- GABA receptor binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI]
- protein C-terminus binding [IPI, ISO]
- protein antigen binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein dimerization activity [IMP]
- protein domain specific binding [IDA]
- protein heterodimerization activity [ISS]
- protein kinase B binding [IPI]
- protein kinase binding [IDA]
- protein phosphatase 2A binding [IDA, IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, IMP, TAS]
- tau protein binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Alpha-synuclein activation of protein phosphatase 2A reduces tyrosine hydroxylase phosphorylation in dopaminergic cells.
alpha-Synuclein is an abundant presynaptic protein implicated in neuronal plasticity and neurodegenerative diseases. Although the function of alpha-synuclein is not thoroughly elucidated, we found that alpha-synuclein regulates dopamine synthesis by binding to and inhibiting tyrosine hydroxylase, the rate limiting enzyme in dopamine synthesis. Understanding alpha-synuclein function in dopaminergic cells should add to our knowledge of this key protein, which is ... [more]
J. Cell. Sci. Aug. 01, 2005; 118(0);3523-30 [Pubmed: 16030137]
Throughput
- Low Throughput
Additional Notes
- figure 4.
Curated By
- BioGRID