RAF1
Gene Ontology Biological Process
- MAPK cascade [IDA, IMP]
- activation of MAPKK activity [IDA, IMP, ISO, TAS]
- cell differentiation [ISO]
- death-inducing signaling complex assembly [ISO]
- heart development [IEP]
- intermediate filament cytoskeleton organization [ISO]
- intracellular signal transduction [TAS]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- negative regulation of protein complex assembly [ISO]
- neurotrophin TRK receptor signaling pathway [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- protein phosphorylation [IDA, IMP, TAS]
- response to hypoxia [IDA]
- response to muscle stretch [ISO]
- somatic stem cell maintenance [ISO]
Gene Ontology Molecular Function- ATP binding [IDA]
- MAP kinase kinase kinase activity [IDA, IMP, TAS]
- Ras GTPase binding [ISO]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein serine/threonine kinase activity [ISO]
- small GTPase binding [ISO]
- ATP binding [IDA]
- MAP kinase kinase kinase activity [IDA, IMP, TAS]
- Ras GTPase binding [ISO]
- identical protein binding [ISO]
- mitogen-activated protein kinase kinase binding [IMP]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein serine/threonine kinase activity [ISO]
- small GTPase binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO, TAS]
- plasma membrane [IDA, ISO]
- pseudopodium [ISO]
PEBP1
Gene Ontology Biological Process
- MAPK cascade [IDA]
- aging [IEP]
- brain development [IEP]
- eating behavior [IEP]
- negative regulation of MAPK cascade [IMP]
- negative regulation of protein phosphorylation [IMP]
- positive regulation of acetylcholine metabolic process [IDA]
- positive regulation of cAMP-mediated signaling [IMP]
- positive regulation of mitosis [IMP]
- regulation of neurotransmitter levels [IDA]
- regulation of the force of heart contraction [IDA]
- response to activity [IEP]
- response to cAMP [IEP]
- response to calcium ion [IEP]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to electrical stimulus [IEP]
- response to ethanol [IEP]
- response to heat [IEP]
- response to organic cyclic compound [IEP]
- response to organic substance [IEP]
- response to organonitrogen compound [IEP]
- response to oxidative stress [IEP]
- response to toxic substance [IEP]
- response to wounding [IEP]
- sperm capacitation [ISO]
- spermatid development [IEP, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- apical part of cell [IDA]
- axon terminus [IDA]
- cell surface [IDA, ISO]
- cytoplasm [IDA]
- extracellular space [IDA]
- extracellular vesicular exosome [ISO]
- mitochondrial outer membrane [IDA]
- mitochondrion [IDA]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO]
- rough endoplasmic reticulum [IDA]
- synaptic vesicle [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Activation of Raf-1 signaling by protein kinase C through a mechanism involving Raf kinase inhibitory protein.
Protein kinase C (PKC) regulates activation of the Raf-1 signaling cascade by growth factors, but the mechanism by which this occurs has not been elucidated. Here we report that one mechanism involves dissociation of Raf kinase inhibitory protein (RKIP) from Raf-1. Classic and atypical but not novel PKC isoforms phosphorylate RKIP at serine 153 (Ser-153). RKIP Ser-153 phosphorylation by PKC ... [more]
Throughput
- Low Throughput
Additional Notes
- kinase activity inhibited by RKIP
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAF1 PEBP1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 735590 |
Curated By
- BioGRID