BAIT
BAX
BCL2-associated X protein
GO Process (107)
GO Function (11)
GO Component (18)
Gene Ontology Biological Process
- B cell apoptotic process [IGI, ISO]
- B cell homeostasis [IGI, IMP]
- B cell homeostatic proliferation [IMP]
- B cell negative selection [IGI]
- B cell receptor apoptotic signaling pathway [ISO]
- Sertoli cell proliferation [IGI]
- T cell homeostatic proliferation [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, IGI, IMP, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [IDA, IMP, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [IMP]
- apoptotic mitochondrial changes [IDA, ISO]
- apoptotic process [IDA, IMP, ISO]
- apoptotic process involved in embryonic digit morphogenesis [IGI]
- apoptotic process involved in patterning of blood vessels [IGI]
- apoptotic signaling pathway [IMP, ISO]
- blood vessel remodeling [IGI]
- cell proliferation [IDA, IGI]
- cellular respiration [ISO]
- cellular response to DNA damage stimulus [IMP]
- cellular response to UV [IGI]
- cellular response to organic substance [IDA]
- cerebral cortex development [IMP]
- development of secondary sexual characteristics [IMP]
- ectopic germ cell programmed cell death [IGI, IMP]
- establishment or maintenance of transmembrane electrochemical gradient [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IGI]
- extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- fertilization [IGI]
- germ cell development [IGI]
- glycosphingolipid metabolic process [IMP]
- homeostasis of number of cells [IGI, IMP]
- homeostasis of number of cells within a tissue [IMP]
- hypothalamus development [IMP]
- inner mitochondrial membrane organization [ISO]
- intrinsic apoptotic signaling pathway [ISO]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IGI, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IGI, ISO]
- kidney development [IGI]
- leukocyte homeostasis [IGI]
- limb morphogenesis [IGI]
- male gonad development [IGI, IMP]
- mitochondrial fragmentation involved in apoptotic process [ISO]
- mitochondrial fusion [IGI, ISO]
- mitochondrion morphogenesis [IGI]
- myeloid cell homeostasis [IGI]
- negative regulation of apoptotic signaling pathway [IGI]
- negative regulation of cell proliferation [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IGI]
- negative regulation of fibroblast proliferation [IMP]
- negative regulation of neuron apoptotic process [IDA]
- negative regulation of peptidyl-serine phosphorylation [IGI]
- negative regulation of protein binding [ISO]
- nervous system development [IMP]
- neuron apoptotic process [IGI, IMP]
- neuron migration [IMP]
- odontogenesis of dentin-containing tooth [IGI]
- outer mitochondrial membrane organization [ISO]
- ovarian follicle development [IGI, IMP]
- positive regulation of B cell apoptotic process [IGI]
- positive regulation of apoptotic DNA fragmentation [ISO]
- positive regulation of apoptotic process [IDA, IGI, IMP, ISO]
- positive regulation of apoptotic process involved in mammary gland involution [IMP]
- positive regulation of calcium ion transport into cytosol [IGI]
- positive regulation of developmental pigmentation [IGI]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [IMP, ISO]
- positive regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- positive regulation of neuron apoptotic process [IGI, IMP, ISO]
- positive regulation of protein oligomerization [ISO]
- positive regulation of release of cytochrome c from mitochondria [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [IMP]
- post-embryonic camera-type eye morphogenesis [IGI]
- post-embryonic development [IGI]
- protein homooligomerization [IDA, ISO]
- protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [IMP]
- protein oligomerization [ISO]
- regulation of cell cycle [IGI]
- regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- regulation of mammary gland epithelial cell proliferation [IMP]
- regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- regulation of mitochondrial membrane permeability involved in programmed necrotic cell death [IMP]
- regulation of mitochondrial membrane potential [IDA]
- regulation of neuron apoptotic process [IMP]
- regulation of nitrogen utilization [IGI]
- regulation of protein heterodimerization activity [ISO]
- regulation of protein homodimerization activity [ISO]
- release of cytochrome c from mitochondria [IDA, IGI, ISO]
- release of matrix enzymes from mitochondria [ISO]
- response to axon injury [IMP]
- response to gamma radiation [IMP]
- response to ionizing radiation [IGI, IMP]
- response to salt stress [IGI]
- response to toxic substance [IMP, ISO]
- response to wounding [IMP]
- retina development in camera-type eye [IMP]
- retinal cell apoptotic process [ISO]
- retinal cell programmed cell death [IMP]
- sex differentiation [IMP]
- spermatid differentiation [IMP]
- spermatogenesis [IGI, IMP]
- thymocyte apoptotic process [IGI]
- transformed cell apoptotic process [ISO]
- transmembrane transport [IBA]
- vagina development [IGI]
Gene Ontology Molecular Function- BH domain binding [ISO]
- BH3 domain binding [ISO]
- channel activity [IBA, ISO]
- chaperone binding [ISO]
- heat shock protein binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IMP, IPI, ISO]
- BH domain binding [ISO]
- BH3 domain binding [ISO]
- channel activity [IBA, ISO]
- chaperone binding [ISO]
- heat shock protein binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IMP, IPI, ISO]
Gene Ontology Cellular Component
- BAX complex [IPI, ISO]
- Bcl-2 family protein complex [ISO]
- cell [IGI]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [IDA, IGI, ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISS]
- intracellular [IDA, IGI]
- membrane [ISO]
- mitochondrial membrane [IDA, ISO]
- mitochondrial outer membrane [IDA, ISO]
- mitochondrial permeability transition pore complex [ISO]
- mitochondrion [IDA, IGI, ISO]
- nuclear envelope [ISO]
- nucleus [ISO]
- pore complex [ISO]
Mus musculus
PREY
DNAJA1
DJ-2, DjA1, HDJ2, HSDJ, HSJ2, HSPF4, NEDD7, hDJ-2, RP11-54K16.1
DnaJ (Hsp40) homolog, subfamily A, member 1
GO Process (9)
GO Function (7)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of JUN kinase activity [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of protein ubiquitination [IDA]
- positive regulation of apoptotic process [IMP]
- protein folding [TAS]
- protein localization to mitochondrion [IMP]
- protein refolding [IBA]
- regulation of protein transport [IDA]
- response to unfolded protein [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria.
We reported that the endoplasmic reticulum (ER) stress pathway involving CHOP, a member of the C/EBP transcription factor family, plays a key role in nitric oxide (NO)-mediated apoptosis of macrophages and pancreatic beta cells. We also showed that the cytosolic chaperone pair of hsp70 and dj1 (hsp40/hdj-1) or dj2 (HSDJ/hdj-2) prevents NO-mediated apoptosis upstream of cytochrome c release from mitochondria. ... [more]
Cell Death Differ. Apr. 01, 2004; 11(4);390-402 [Pubmed: 14752510]
Throughput
- Low Throughput
Additional Notes
- figure 10.
Curated By
- BioGRID