BAIT

CUL3

AI467304, AW146203, mKIAA0617
cullin 3
GO Process (26)
GO Function (6)
GO Component (8)
Mus musculus
PREY

CUL3

AI467304, AW146203, mKIAA0617
cullin 3
GO Process (26)
GO Function (6)
GO Component (8)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.

Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A

The differentiation of several T- and B-cell effector programs in the immune system is directed by signature transcription factors that induce rapid epigenetic remodelling. Here we report that promyelocytic leukaemia zinc finger (PLZF), the BTB-zinc finger (BTB-ZF) transcription factor directing the innate-like effector program of natural killer T-cell thymocytes, is prominently associated with cullin 3 (CUL3), an E3 ubiquitin ligase ... [more]

Nature Nov. 22, 2012; 491(7425);618-21 [Pubmed: 23086144]

Throughput

  • Low Throughput

Curated By

  • BioGRID