BAIT
SNCA
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (70)
GO Function (26)
GO Component (25)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- adult locomotory behavior [ISO]
- aging [IEP]
- behavioral response to cocaine [IMP]
- calcium ion homeostasis [ISO]
- cellular response to copper ion [ISO]
- cellular response to fibroblast growth factor stimulus [IEP]
- cellular response to oxidative stress [ISO]
- dopamine biosynthetic process [ISO]
- dopamine metabolic process [ISO]
- fatty acid metabolic process [ISO]
- long-term synaptic potentiation [ISO]
- membrane organization [ISO]
- microglial cell activation [ISO]
- mitochondrial ATP synthesis coupled electron transport [ISO]
- mitochondrial membrane organization [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of dopamine metabolic process [IMP]
- negative regulation of dopamine uptake involved in synaptic transmission [ISO]
- negative regulation of exocytosis [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of microtubule polymerization [ISO]
- negative regulation of monooxygenase activity [ISO]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of norepinephrine uptake [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of serotonin uptake [ISO]
- negative regulation of thrombin receptor signaling pathway [ISO]
- negative regulation of transporter activity [ISO]
- neutral lipid metabolic process [ISO]
- oxidation-reduction process [ISO]
- phospholipid metabolic process [ISO]
- positive regulation of endocytosis [ISO]
- positive regulation of glutathione peroxidase activity [ISO]
- positive regulation of hydrogen peroxide catabolic process [ISO]
- positive regulation of inositol phosphate biosynthetic process [ISO]
- positive regulation of neurotransmitter secretion [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of receptor recycling [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission [ISO]
- protein destabilization [ISO]
- receptor internalization [ISO]
- regulation of acyl-CoA biosynthetic process [ISO]
- regulation of dopamine secretion [ISO]
- regulation of excitatory postsynaptic membrane potential [ISO]
- regulation of glutamate secretion [ISO]
- regulation of locomotion [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of macrophage activation [ISO]
- regulation of neuron apoptotic process [ISO]
- regulation of neuron death [IGI]
- regulation of neuronal synaptic plasticity [ISO]
- regulation of neurotransmitter secretion [ISO]
- regulation of phospholipase activity [ISO]
- response to cocaine [IEP]
- response to drug [ISO]
- response to interferon-gamma [ISO]
- response to interleukin-1 [ISO]
- response to iron(II) ion [ISO]
- response to lipopolysaccharide [ISO]
- response to magnesium ion [ISO]
- synapse organization [ISO]
- synaptic transmission [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic vesicle endocytosis [ISO]
- synaptic vesicle transport [ISO]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [ISO]
- beta-tubulin binding [IDA]
- calcium ion binding [ISO]
- copper ion binding [ISO, ISS]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [IPI]
- fatty acid binding [ISO]
- ferrous iron binding [ISO]
- histone binding [ISO]
- identical protein binding [ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [IDA]
- oxidoreductase activity [ISO]
- phospholipase D inhibitor activity [ISO]
- phospholipase binding [IPI]
- phospholipid binding [IDA, ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- tau protein binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- actin cytoskeleton [ISO]
- axon [ISO]
- axon terminus [IDA]
- cell [ISO]
- cell cortex [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle membrane [IDA]
- cytoskeleton [ISO]
- cytosol [IDA, ISO]
- fibril [ISO]
- growth cone [IDA, ISO]
- inclusion body [ISO]
- intracellular membrane-bounded organelle [IDA]
- mitochondrion [IDA, ISO]
- nuclear outer membrane [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- platelet alpha granule membrane [ISO]
- ribosome [IDA]
- rough endoplasmic reticulum [IDA]
- synapse [ISO]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
Rattus norvegicus
PREY
HSPA8
Hsc70
heat shock 70kDa protein 8
GO Process (10)
GO Function (13)
GO Component (18)
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- chaperone mediated protein folding requiring cofactor [IDA, ISO]
- chaperone-mediated protein folding [IDA]
- clathrin coat disassembly [ISO]
- negative regulation of fibril organization [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of mRNA splicing, via spliceosome [ISO]
- protein folding [ISO]
- protein refolding [ISO]
- regulation of cell cycle [ISO]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
Gene Ontology Cellular Component
- Prp19 complex [ISO, ISS]
- blood microparticle [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- focal adhesion [ISO]
- intracellular [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO, ISS]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
- ubiquitin ligase complex [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of novel proteins associated with both alpha-synuclein and DJ-1.
The molecular mechanisms leading to neurodegeneration in Parkinson disease (PD) remain elusive, although many lines of evidence have indicated that alpha-synuclein and DJ-1, two critical proteins in PD pathogenesis, interact with each other functionally. The investigation on whether alpha-synuclein directly interacts with DJ-1 has been controversial. In the current study, we analyzed proteins associated with alpha-synuclein and/or DJ-1 with a ... [more]
Mol. Cell Proteomics May. 01, 2007; 6(5);845-59 [Pubmed: 16854843]
Throughput
- High Throughput
Additional Notes
- appendix I.
Curated By
- BioGRID