BAIT
BAX
BCL2-associated X protein
GO Process (107)
GO Function (11)
GO Component (18)
Gene Ontology Biological Process
- B cell apoptotic process [IGI, ISO]
- B cell homeostasis [IGI, IMP]
- B cell homeostatic proliferation [IMP]
- B cell negative selection [IGI]
- B cell receptor apoptotic signaling pathway [ISO]
- Sertoli cell proliferation [IGI]
- T cell homeostatic proliferation [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, IGI, IMP, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [IDA, IMP, ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [IMP]
- apoptotic mitochondrial changes [IDA, ISO]
- apoptotic process [IDA, IMP, ISO]
- apoptotic process involved in embryonic digit morphogenesis [IGI]
- apoptotic process involved in patterning of blood vessels [IGI]
- apoptotic signaling pathway [IMP, ISO]
- blood vessel remodeling [IGI]
- cell proliferation [IDA, IGI]
- cellular respiration [ISO]
- cellular response to DNA damage stimulus [IMP]
- cellular response to UV [IGI]
- cellular response to organic substance [IDA]
- cerebral cortex development [IMP]
- development of secondary sexual characteristics [IMP]
- ectopic germ cell programmed cell death [IGI, IMP]
- establishment or maintenance of transmembrane electrochemical gradient [ISO]
- extrinsic apoptotic signaling pathway [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IGI]
- extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- fertilization [IGI]
- germ cell development [IGI]
- glycosphingolipid metabolic process [IMP]
- homeostasis of number of cells [IGI, IMP]
- homeostasis of number of cells within a tissue [IMP]
- hypothalamus development [IMP]
- inner mitochondrial membrane organization [ISO]
- intrinsic apoptotic signaling pathway [ISO]
- intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to DNA damage [IGI, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IGI, ISO]
- kidney development [IGI]
- leukocyte homeostasis [IGI]
- limb morphogenesis [IGI]
- male gonad development [IGI, IMP]
- mitochondrial fragmentation involved in apoptotic process [ISO]
- mitochondrial fusion [IGI, ISO]
- mitochondrion morphogenesis [IGI]
- myeloid cell homeostasis [IGI]
- negative regulation of apoptotic signaling pathway [IGI]
- negative regulation of cell proliferation [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IGI]
- negative regulation of fibroblast proliferation [IMP]
- negative regulation of neuron apoptotic process [IDA]
- negative regulation of peptidyl-serine phosphorylation [IGI]
- negative regulation of protein binding [ISO]
- nervous system development [IMP]
- neuron apoptotic process [IGI, IMP]
- neuron migration [IMP]
- odontogenesis of dentin-containing tooth [IGI]
- outer mitochondrial membrane organization [ISO]
- ovarian follicle development [IGI, IMP]
- positive regulation of B cell apoptotic process [IGI]
- positive regulation of apoptotic DNA fragmentation [ISO]
- positive regulation of apoptotic process [IDA, IGI, IMP, ISO]
- positive regulation of apoptotic process involved in mammary gland involution [IMP]
- positive regulation of calcium ion transport into cytosol [IGI]
- positive regulation of developmental pigmentation [IGI]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [IMP, ISO]
- positive regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- positive regulation of neuron apoptotic process [IGI, IMP, ISO]
- positive regulation of protein oligomerization [ISO]
- positive regulation of release of cytochrome c from mitochondria [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [IMP]
- post-embryonic camera-type eye morphogenesis [IGI]
- post-embryonic development [IGI]
- protein homooligomerization [IDA, ISO]
- protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [IMP]
- protein oligomerization [ISO]
- regulation of cell cycle [IGI]
- regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- regulation of mammary gland epithelial cell proliferation [IMP]
- regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- regulation of mitochondrial membrane permeability involved in programmed necrotic cell death [IMP]
- regulation of mitochondrial membrane potential [IDA]
- regulation of neuron apoptotic process [IMP]
- regulation of nitrogen utilization [IGI]
- regulation of protein heterodimerization activity [ISO]
- regulation of protein homodimerization activity [ISO]
- release of cytochrome c from mitochondria [IDA, IGI, ISO]
- release of matrix enzymes from mitochondria [ISO]
- response to axon injury [IMP]
- response to gamma radiation [IMP]
- response to ionizing radiation [IGI, IMP]
- response to salt stress [IGI]
- response to toxic substance [IMP, ISO]
- response to wounding [IMP]
- retina development in camera-type eye [IMP]
- retinal cell apoptotic process [ISO]
- retinal cell programmed cell death [IMP]
- sex differentiation [IMP]
- spermatid differentiation [IMP]
- spermatogenesis [IGI, IMP]
- thymocyte apoptotic process [IGI]
- transformed cell apoptotic process [ISO]
- transmembrane transport [IBA]
- vagina development [IGI]
Gene Ontology Molecular Function- BH domain binding [ISO]
- BH3 domain binding [ISO]
- channel activity [IBA, ISO]
- chaperone binding [ISO]
- heat shock protein binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IMP, IPI, ISO]
- BH domain binding [ISO]
- BH3 domain binding [ISO]
- channel activity [IBA, ISO]
- chaperone binding [ISO]
- heat shock protein binding [ISO]
- identical protein binding [ISO]
- lipid binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [IPI, ISO]
- protein homodimerization activity [IMP, IPI, ISO]
Gene Ontology Cellular Component
- BAX complex [IPI, ISO]
- Bcl-2 family protein complex [ISO]
- cell [IGI]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- endoplasmic reticulum [IDA, IGI, ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISS]
- intracellular [IDA, IGI]
- membrane [ISO]
- mitochondrial membrane [IDA, ISO]
- mitochondrial outer membrane [IDA, ISO]
- mitochondrial permeability transition pore complex [ISO]
- mitochondrion [IDA, IGI, ISO]
- nuclear envelope [ISO]
- nucleus [ISO]
- pore complex [ISO]
Mus musculus
PREY
PARK2
PRKN
Parkinson disease (autosomal recessive, juvenile) 2, parkin
GO Process (48)
GO Function (21)
GO Component (21)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- aggresome assembly [ISO]
- cellular protein catabolic process [ISO]
- cellular response to toxic substance [ISO]
- dopamine metabolic process [IMP]
- dopamine uptake involved in synaptic transmission [IMP]
- learning [IMP]
- locomotory behavior [IMP]
- mitochondrion degradation [ISO]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO]
- negative regulation of glucokinase activity [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of neuron death [ISO]
- negative regulation of oxidative stress-induced neuron death [ISO]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of reactive oxygen species metabolic process [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- norepinephrine metabolic process [IMP]
- positive regulation of DNA binding [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of mitochondrial fusion [ISO]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- protein metabolic process [IMP]
- protein monoubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of autophagy [ISO]
- regulation of mitochondrion organization [ISO]
- regulation of neurotransmitter secretion [IMP]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [IMP]
- response to endoplasmic reticulum stress [ISO]
- response to unfolded protein [ISO]
- startle response [IMP]
- synaptic transmission, dopaminergic [IMP]
- synaptic transmission, glutamatergic [IMP]
Gene Ontology Molecular Function- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [ISO]
- LUBAC complex [ISO]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO]
- aggresome [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- intracellular [ISO]
- membrane raft [ISO]
- mitochondrion [ISO]
- neuron projection [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [ISO]
- protein complex [IDA]
- synapse [ISO]
- synaptic vesicle [ISO]
- ubiquitin ligase complex [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The ubiquitin E3 ligase parkin regulates the proapoptotic function of Bax.
Autosomal recessive loss-of-function mutations within the PARK2 gene functionally inactivate the E3 ubiquitin ligase parkin, resulting in neurodegeneration of catecholaminergic neurons and a familial form of Parkinson disease. Current evidence suggests both a mitochondrial function for parkin and a neuroprotective role, which may in fact be interrelated. The antiapoptotic effects of parkin have been widely reported, and may involve fundamental ... [more]
Proc. Natl. Acad. Sci. U.S.A. Apr. 17, 2012; 109(16);6283-8 [Pubmed: 22460798]
Throughput
- Low Throughput
Additional Notes
- figure 3.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PARK2 BAX | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 736309 |
Curated By
- BioGRID