BAIT

WBP11

2510026P17Rik, D6Wsu113e, Npwbp, SIPP1
WW domain binding protein 11
GO Process (2)
GO Function (4)
GO Component (5)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

SIPP1, a novel pre-mRNA splicing factor and interactor of protein phosphatase-1.

Llorian M, Beullens M, Andres I, Ortiz JM, Bollen M

We have identified a polypeptide that was already known to interact with polyglutamine-tract-binding protein (PQBP)-1/Npw38 as a novel splicing factor and interactor of protein phosphatase-1, hence the name SIPP1 for splicing factor that interacts with PQBP-1 and PP1 (protein phosphotase 1). SIPP1 was inhibitory to PP1, and its inhibitory potency was increased by phosphorylation with protein kinase CK1. Two-hybrid and ... [more]

Biochem. J. Feb. 15, 2004; 378(0);229-38 [Pubmed: 14640981]

Throughput

  • Low Throughput

Curated By

  • BioGRID