An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death.

Imai Y, Soda M, Murakami T, Shoji M, Abe K, Takahashi R

Parkin, a RING-type ubiquitin ligase, is the product of the gene responsible for autosomal recessive juvenile parkinsonism. A reverse strand gene located upstream of the parkin gene in the human genome has been identified. The gene product, termed Glup/PACRG, forms a large molecular chaperone complex containing heat shock proteins 70 and 90 and chaperonin components. Glup suppressed cell death induced ... [more]

J. Biol. Chem. Dec. 19, 2003; 278(51);51901-10 [Pubmed: 14532270]

Throughput

Low Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Additional Notes

exogenous expression of bait
figure 1.

Curated By

BioGRID

Interaction Summary

PACRG

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled receptor binding [IPI]Hsp70 protein binding [IPI]Hsp90 protein binding [IDA]actin binding [IDA]alpha-tubulin binding [IDA]beta-tubulin binding [IDA]chaperone binding [IPI]heat shock protein binding [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

CCT3

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]