BAIT
SLC6A3
DAT, Dat1
solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
GO Process (17)
GO Function (11)
GO Component (4)
Gene Ontology Biological Process
- adenohypophysis development [IMP]
- cation transmembrane transport [IMP, ISO]
- dopamine biosynthetic process [IMP]
- dopamine catabolic process [IMP]
- dopamine transport [IMP, ISO]
- lactation [IMP]
- locomotory behavior [IGI, IMP]
- monoamine transport [ISO]
- neurotransmitter transport [ISO]
- positive regulation of multicellular organism growth [IMP]
- prepulse inhibition [IMP]
- regulation of dopamine metabolic process [IMP]
- response to cAMP [ISO]
- response to cocaine [IDA]
- response to ethanol [ISO]
- sensory perception of smell [IMP]
- sodium ion transmembrane transport [ISO]
Gene Ontology Molecular Function- dopamine binding [IDA]
- dopamine transmembrane transporter activity [IMP, ISO]
- dopamine:sodium symporter activity [ISO]
- drug binding [ISO]
- monoamine transmembrane transporter activity [ISO]
- protease binding [ISO]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [ISO]
- protein phosphatase 2A binding [ISO]
- receptor binding [ISO]
- dopamine binding [IDA]
- dopamine transmembrane transporter activity [IMP, ISO]
- dopamine:sodium symporter activity [ISO]
- drug binding [ISO]
- monoamine transmembrane transporter activity [ISO]
- protease binding [ISO]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [ISO]
- protein phosphatase 2A binding [ISO]
- receptor binding [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
SNCA
NACP, alphaSYN
synuclein, alpha
GO Process (66)
GO Function (24)
GO Component (25)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- adult locomotory behavior [IGI]
- behavioral response to cocaine [ISO]
- cellular response to copper ion [ISO]
- cellular response to oxidative stress [IMP]
- dopamine biosynthetic process [IMP]
- dopamine metabolic process [IGI]
- fatty acid metabolic process [IMP]
- long-term synaptic potentiation [IMP]
- membrane organization [IMP]
- microglial cell activation [IMP]
- mitochondrial ATP synthesis coupled electron transport [IMP]
- mitochondrial membrane organization [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of dopamine metabolic process [ISO]
- negative regulation of dopamine uptake involved in synaptic transmission [ISO]
- negative regulation of exocytosis [ISO]
- negative regulation of histone acetylation [ISO]
- negative regulation of microtubule polymerization [ISO]
- negative regulation of monooxygenase activity [ISO]
- negative regulation of neuron apoptotic process [IGI, ISO]
- negative regulation of norepinephrine uptake [ISO]
- negative regulation of platelet-derived growth factor receptor signaling pathway [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of serotonin uptake [ISO]
- negative regulation of thrombin receptor signaling pathway [ISO]
- negative regulation of transporter activity [ISO]
- neutral lipid metabolic process [IMP]
- oxidation-reduction process [ISO]
- phospholipid metabolic process [IMP]
- positive regulation of endocytosis [ISO]
- positive regulation of glutathione peroxidase activity [ISO]
- positive regulation of hydrogen peroxide catabolic process [ISO]
- positive regulation of inositol phosphate biosynthetic process [ISO]
- positive regulation of neurotransmitter secretion [IDA]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of receptor recycling [ISO]
- positive regulation of release of sequestered calcium ion into cytosol [ISO]
- positive regulation of synaptic transmission [IMP]
- protein destabilization [ISO]
- receptor internalization [ISO]
- regulation of acyl-CoA biosynthetic process [IDA, IMP]
- regulation of dopamine secretion [IGI]
- regulation of excitatory postsynaptic membrane potential [IMP]
- regulation of glutamate secretion [IMP]
- regulation of locomotion [IMP]
- regulation of long-term neuronal synaptic plasticity [IMP]
- regulation of macrophage activation [IMP]
- regulation of neuron apoptotic process [IMP]
- regulation of neuron death [ISO]
- regulation of neuronal synaptic plasticity [IMP]
- regulation of neurotransmitter secretion [IGI, IMP]
- regulation of phospholipase activity [ISO]
- response to drug [IMP]
- response to interferon-gamma [ISO]
- response to interleukin-1 [ISO]
- response to iron(II) ion [ISO]
- response to lipopolysaccharide [ISO]
- response to magnesium ion [ISO]
- synapse organization [IGI]
- synaptic transmission [IGI]
- synaptic transmission, dopaminergic [IMP]
- synaptic vesicle endocytosis [IMP]
- synaptic vesicle transport [IMP]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [IDA]
- beta-tubulin binding [ISO]
- calcium ion binding [ISO]
- copper ion binding [ISO]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [ISO]
- ferrous iron binding [ISO]
- histone binding [IDA, ISO]
- identical protein binding [IDA, ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [ISO]
- oxidoreductase activity [ISO]
- phospholipase binding [ISO]
- phospholipid binding [ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- tau protein binding [ISO]
- zinc ion binding [ISO]
- Hsp70 protein binding [ISO]
- alpha-tubulin binding [ISO]
- arachidonic acid binding [IDA]
- beta-tubulin binding [ISO]
- calcium ion binding [ISO]
- copper ion binding [ISO]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [ISO]
- dynein binding [ISO]
- enzyme binding [ISO]
- ferrous iron binding [ISO]
- histone binding [IDA, ISO]
- identical protein binding [IDA, ISO]
- kinesin binding [ISO]
- magnesium ion binding [ISO]
- microtubule binding [ISO]
- oxidoreductase activity [ISO]
- phospholipase binding [ISO]
- phospholipid binding [ISO]
- phosphoprotein binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- tau protein binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- actin cytoskeleton [ISO]
- axon [ISO]
- axon terminus [ISO]
- cell [IMP]
- cell cortex [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle membrane [ISO]
- cytoskeleton [IDA]
- cytosol [IDA, ISO]
- fibril [ISO]
- growth cone [ISO]
- inclusion body [IDA, ISO]
- intracellular membrane-bounded organelle [ISO]
- mitochondrion [IMP, ISO]
- nuclear outer membrane [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- platelet alpha granule membrane [ISO]
- ribosome [ISO]
- rough endoplasmic reticulum [ISO]
- synapse [IDA]
- synaptic vesicle [ISO]
- terminal bouton [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
α-Synuclein stimulates a dopamine transporter-dependent chloride current and modulates the activity of the transporter.
Dysregulation of dopamine (DA) homeostasis is implicated in neurodegenerative diseases, drug addiction, and neuropsychiatric disorders. The neuronal plasma membrane dopamine transporter (DAT) is essential for the maintenance of DA homeostasis in the brain. α-Synuclein is a 140-amino acid protein that forms a stable complex with DAT and is linked to the pathogenesis of neurodegenerative disease. To elucidate the potential functional ... [more]
J. Biol. Chem. Dec. 23, 2011; 286(51);43933-43 [Pubmed: 21990355]
Throughput
- Low Throughput
Additional Notes
- figure S1.
Curated By
- BioGRID