RPS6KA5
Gene Ontology Biological Process
- histone H2A-S1 phosphorylation [ISO]
- histone H3-S10 phosphorylation [ISO, ISS]
- histone H3-S28 phosphorylation [ISO, ISS]
- histone phosphorylation [ISO]
- interleukin-1-mediated signaling pathway [ISO]
- intracellular signal transduction [IDA, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of histone acetylation [ISO]
- positive regulation of histone phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
Gene Ontology Molecular Function
YWHAZ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex.
Upon activation of the ERK and p38 MAPK pathways, the MSK1/2-mediated nucleosomal response, including H3 phosphorylation at serine 28 or 10, is coupled with the induction of immediate-early (IE) gene transcription. The outcome of this response, varying with the stimuli and cellular contexts, ranges from neoplastic transformation to neuronal synaptic plasticity. Here, we used sequential co-immunoprecipitation assays and sequential chromatin ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YWHAZ RPS6KA5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID