HIF1A
Gene Ontology Biological Process
- B-1 B cell homeostasis [IMP]
- angiogenesis [IMP]
- axon transport of mitochondrion [ISO]
- blood vessel development [IMP]
- blood vessel morphogenesis [IMP]
- cardiac ventricle morphogenesis [IMP]
- cartilage development [IMP]
- cell differentiation [IGI, IMP]
- cellular iron ion homeostasis [IMP]
- cellular response to hypoxia [IGI, ISO]
- cerebral cortex development [IMP]
- collagen metabolic process [IMP]
- connective tissue replacement involved in inflammatory response wound healing [IMP]
- digestive tract morphogenesis [IMP]
- dopaminergic neuron differentiation [IGI, IMP]
- elastin metabolic process [IMP]
- embryonic hemopoiesis [IMP]
- embryonic placenta development [IGI, IMP]
- epithelial cell differentiation involved in mammary gland alveolus development [IMP]
- epithelial to mesenchymal transition [IMP]
- glucose homeostasis [IMP]
- heart looping [IMP]
- hemoglobin biosynthetic process [IMP]
- hypoxia-inducible factor-1alpha signaling pathway [IDA]
- intestinal epithelial cell maturation [IMP]
- lactate metabolic process [IMP]
- lactation [IMP]
- muscle cell cellular homeostasis [IMP]
- negative regulation of TOR signaling [IMP]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of bone mineralization [IMP]
- negative regulation of growth [IMP]
- negative regulation of mesenchymal cell apoptotic process [IMP]
- negative regulation of neuron apoptotic process [IMP]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO]
- negative regulation of thymocyte apoptotic process [IMP]
- negative regulation of transcription elongation from RNA polymerase II promoter [ISO]
- negative regulation of vasoconstriction [ISO]
- neural crest cell migration [IMP]
- neural fold elevation formation [IMP]
- outflow tract morphogenesis [IMP]
- oxygen homeostasis [IMP, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of epithelial cell migration [IMP]
- positive regulation of erythrocyte differentiation [IMP]
- positive regulation of gene expression [ISO]
- positive regulation of hormone biosynthetic process [ISO]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [IMP]
- positive regulation of neuroblast proliferation [IGI]
- positive regulation of receptor biosynthetic process [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter in response to hypoxia [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [IMP]
- positive regulation vascular endothelial growth factor production [ISO]
- regulation of catalytic activity [IMP]
- regulation of cell proliferation [IMP]
- regulation of gene expression [ISO]
- regulation of glycolytic process [IGI]
- regulation of thymocyte apoptotic process [IGI]
- regulation of transcription from RNA polymerase II promoter [IPI]
- regulation of transcription from RNA polymerase II promoter in response to oxidative stress [IMP, ISO]
- regulation of transcription, DNA-templated [IDA, ISO]
- regulation of transforming growth factor beta2 production [ISO]
- response to fungicide [ISO]
- response to hypoxia [IDA, IGI, IMP, ISO]
- response to muscle activity [IMP]
- retina vasculature development in camera-type eye [IMP]
- signal transduction [ISO]
- transcription from RNA polymerase II promoter [IPI]
- vascular endothelial growth factor production [ISO]
- vasculature development [IGI]
- visual learning [IMP]
Gene Ontology Molecular Function- DNA binding [IDA, IGI]
- Hsp90 protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- ubiquitin protein ligase binding [ISO]
- DNA binding [IDA, IGI]
- Hsp90 protein binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
- transcription factor binding transcription factor activity [ISO]
- ubiquitin protein ligase binding [ISO]
VHL
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [IMP]
- angiogenesis [IMP]
- blood vessel endothelial cell migration [IMP]
- extracellular matrix organization [IMP]
- negative regulation of thymocyte apoptotic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [ISO]
- neuron differentiation [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- proteasomal protein catabolic process [IMP]
- protein catabolic process [IMP]
- protein heterooligomerization [ISO]
- protein transport [IDA]
- protein ubiquitination [ISO]
- regulation of apoptotic signaling pathway [IGI]
- regulation of catecholamine metabolic process [ISO]
- regulation of thymocyte apoptotic process [IGI]
- regulation of transcription, DNA-templated [ISO]
- response to hypoxia [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of residues critical for regulation of protein stability and the transactivation function of the hypoxia-inducible factor-1alpha by the von Hippel-Lindau tumor suppressor gene product.
Under normoxic conditions the hypoxia-inducible factor-1alpha (HIF-1alpha) protein is targeted for degradation by the von Hippel-Lindau (pVHL) tumor suppressor protein acting as an E3 ubiquitin ligase. Binding of pVHL to HIF-1alpha is dependent on hydroxylation of specific proline residues by O(2)-dependent prolyl 4-hydroxylases. Upon exposure to hypoxia the hydroxylase activity is inhibited, resulting in stabilization of HIF-1alpha protein levels and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VHL HIF1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HIF1A VHL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VHL HIF1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HIF1A VHL | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| VHL HIF1A | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| HIF1A VHL | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID