SIRT4
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IDA]
- glutamine metabolic process [IMP]
- negative regulation of cardiac muscle cell apoptotic process [ISO]
- negative regulation of fatty acid oxidation [IDA]
- negative regulation of insulin secretion [IGI, IMP, ISO]
- negative regulation of protein processing involved in protein targeting to mitochondrion [ISO]
- peptidyl-lysine deacetylation [IDA]
- positive regulation of lipid biosynthetic process [IDA]
- protein ADP-ribosylation [IMP]
- tricarboxylic acid metabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GLUD1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SIRT4 inhibits glutamate dehydrogenase and opposes the effects of calorie restriction in pancreatic beta cells.
Sir2 is an NAD-dependent deacetylase that connects metabolism with longevity in yeast, flies, and worms. Mammals have seven Sir2 homologs (SIRT1-7). We show that SIRT4 is a mitochondrial enzyme that uses NAD to ADP-ribosylate and downregulate glutamate dehydrogenase (GDH) activity. GDH is known to promote the metabolism of glutamate and glutamine, generating ATP, which promotes insulin secretion. Loss of SIRT4 ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID