ILK
Gene Ontology Biological Process
- Schwann cell development [IMP]
- branching involved in ureteric bud morphogenesis [IDA]
- cell aging [ISO]
- cell cycle arrest [ISO]
- cell projection organization [IMP]
- establishment or maintenance of epithelial cell apical/basal polarity [IMP]
- extracellular fibril organization [ISO]
- fibroblast migration [IMP]
- integrin-mediated signaling pathway [ISO]
- myelin assembly [ISO]
- myelination in peripheral nervous system [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of cardiac muscle cell apoptotic process [ISO]
- negative regulation of neural precursor cell proliferation [IGI, IMP]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of protein kinase activity [ISO]
- negative regulation of smooth muscle cell migration [ISO]
- negative regulation of smooth muscle cell proliferation [ISO]
- nerve development [IMP]
- neuron projection morphogenesis [ISO]
- peptidyl-serine phosphorylation [IMP, ISO]
- platelet aggregation [ISO]
- positive regulation of BMP signaling pathway [IMP]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of axon extension [ISO]
- positive regulation of axonogenesis [ISO]
- positive regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [IMP, ISO]
- positive regulation of cell-matrix adhesion [ISO]
- positive regulation of dendrite morphogenesis [ISO]
- positive regulation of myoblast differentiation [ISO]
- positive regulation of osteoblast differentiation [IMP]
- positive regulation of phosphorylation [ISO]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- protein heterooligomerization [ISO]
- protein kinase B signaling [IMP]
- protein phosphorylation [ISO]
- regulation of actin cytoskeleton organization [ISO]
- regulation of cell growth [ISO]
- substrate adhesion-dependent cell spreading [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- axon [ISO]
- cell junction [ISO]
- cell-cell junction [ISO]
- costamere [ISO]
- cytoplasm [ISO]
- dendritic shaft [ISO]
- extracellular matrix [ISO]
- focal adhesion [IDA, ISA, ISO]
- intracellular [IMP]
- lamellipodium [IDA]
- membrane [ISO]
- neuronal cell body [ISO]
- nucleoplasm [ISO]
- protein complex [ISO]
- stress fiber [ISO]
- terminal bouton [ISO]
LIMS1
Gene Ontology Biological Process
- cell-cell junction organization [IGI]
- cell-matrix adhesion [IMP]
- chordate embryonic development [IMP]
- establishment of protein localization [ISO]
- establishment or maintenance of cell polarity [IMP]
- negative regulation of apoptotic process [IGI]
- negative regulation of epithelial cell proliferation [IGI]
- negative regulation of hepatocyte proliferation [IGI]
- negative regulation of neural precursor cell proliferation [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of GTPase activity [ISO]
- positive regulation of cell-substrate adhesion [ISO]
- positive regulation of focal adhesion assembly [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of integrin-mediated signaling pathway [IGI]
- positive regulation of substrate adhesion-dependent cell spreading [ISO]
- protein heterooligomerization [ISO]
- single organismal cell-cell adhesion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites.
PINCH, integrin-linked kinase (ILK) and calponin homology-containing ILK-binding protein (CH-ILKBP) form a ternary complex that plays crucial roles at cell-extracellular matrix adhesion sites. To understand the mechanism underlying the complex formation and recruitment to cell-adhesion sites we have undertaken a combined structural, mutational and cell biological analysis. Three-dimensional structure-based point mutations identified specific PINCH and ILK sites that mediate the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ILK LIMS1 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | Low | - | BioGRID | - | |
| ILK LIMS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ILK LIMS1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| LIMS1 ILK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ILK LIMS1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID