BAIT

SNCA

NACP, PARK1, PARK4, PD1
synuclein, alpha (non A4 component of amyloid precursor)
GO Process (45)
GO Function (20)
GO Component (16)

Gene Ontology Biological Process

Homo sapiens
PREY

TUBB1

tubulin, beta 1 class VI
GO Process (3)
GO Function (0)
GO Component (2)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Disruption of the interaction of alpha-synuclein with microtubules enhances cell surface recruitment of the dopamine transporter.

Wersinger C, Sidhu A

Mutations in alpha-synuclein have been implicated in the genesis of Parkinson's disease. A probable normative function of alpha-synuclein is the maintenance of dopamine homeostasis, partly through a negative modulation of dopamine transporter (DAT) activity, by reducing its level at the cell surface. To study the possible involvement of the microtubular network in the alpha-synuclein-dependent trafficking of DAT, we treated cotransfected ... [more]

Biochemistry Oct. 18, 2005; 44(41);13612-24 [Pubmed: 16216085]

Throughput

  • Low Throughput

Additional Notes

  • figure 5.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TUBB1 SNCA
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
736923

Curated By

  • BioGRID