BAIT

EMG1

NEP1, YLR186W
Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity
Saccharomyces cerevisiae (S288c)
PREY

EFG1

YGR272C, YGR271C-A
Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Genetic interactions of yeast NEP1 (EMG1), encoding an essential factor in ribosome biogenesis.

Schilling V, Peifer C, Buchhaupt M, Lamberth S, Lioutikov A, Rietschel B, Koetter P, Entian KD

Nep1 methylates the hypermodified ψ1191 base of 18S rRNA and has an additional essential function during ribosome biogenesis. It is strongly conserved in eukaryotes and a point mutation causes the human Bowen-Conradi syndrome. To identify Δnep1-specific genetic interactions, viable deletions were screened genome-wide (SGA). Due to its essential function, we used, for the first time, query strain (Δnep1) with two ... [more]

Yeast May. 01, 2012; 29(5);167-83 [Pubmed: 22588997]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • nop6 mcRPS19B mutant background (mcRPS19B refers to multi-copy RPS19B)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EFG1 EMG1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID