PREY

NPM1

B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GO Process (23)
GO Function (14)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Thr199 phosphorylation targets nucleophosmin to nuclear speckles and represses pre-mRNA processing.

Tarapore P, Shinmura K, Suzuki H, Tokuyama Y, Kim SH, Mayeda A, Fukasawa K

Nucleophosmin (NPM) is a multifunctional phosphoprotein, being involved in ribosome assembly, pre-ribosomal RNA processing, DNA duplication, nucleocytoplasmic protein trafficking, and centrosome duplication. NPM is phosphorylated by several kinases, including nuclear kinase II, casein kinase 2, Polo-like kinase 1 and cyclin-dependent kinases (CDK1 and 2), and these phosphorylations modulate the activity and function of NPM. We have previously identified Thr(199) as ... [more]

FEBS Lett. Jan. 23, 2006; 580(2);399-409 [Pubmed: 16376875]

Throughput

  • Low Throughput

Additional Notes

  • figure 5.

Curated By

  • BioGRID