A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents.

Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H

To identify novel genes involved in DNA double-strand break (DSB) repair, we previously isolated Schizosaccharomyces pombe mutants which are hypersensitive to methyl methanesulfonate (MMS) and synthetic lethals with rad2. This study characterizes one of these mutants, rad60-1. The gene that complements the MMS sensitivity of this mutant was cloned and designated rad60. rad60 encodes a protein with 406 amino acids ... [more]

Mol. Cell. Biol. May. 01, 2002; 22(10);3537-48 [Pubmed: 11971984]

Throughput

Low Throughput

Ontology Terms

phenotype: resistance to chemicals (APO:0000087)
phenotype: vegetative growth (APO:0000106)

Additional Notes

overxpression of rad60 partially suppresses sensitivity to MMS (CID 4156 CHEBI 25255)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD60 RHP18	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Morishita T (2002)	Low	-	BioGRID	737415

Curated By

BioGRID

Interaction Summary

RHP18

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin protein ligase activity [IMP]

Gene Ontology Cellular Component

RAD60