Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm.

Zhang Y, Zhang H, Liang J, Yu W, Shang Y

Steroid receptor coactivators (SRCs) exert profound effects on animal development and physiology. These coactivators are nuclear proteins and transcription co-regulators that function to facilitate the transcription initiation mediated by nuclear receptors, as well as by other well-known transcription factors. However, how these co-regulators are functionally regulated is poorly understood. During genome-wide screening for SRC-interacting proteins, we identified a novel ankyrin ... [more]

EMBO J. Jun. 06, 2007; 26(11);2645-57 [Pubmed: 17476305]

Throughput

  • Low Throughput

Additional Notes

  • figure 2.

Curated By

  • BioGRID