A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.

Williams DR, McIntosh JR

The fission yeast minichromosome loss mutant mcl1-1 was identified in a screen for mutants defective in chromosome segregation. Missegregation of the chromosomes in mcl1-1 mutant cells results from decreased centromeric cohesion between sister chromatids. mcl1+ encodes a beta-transducin-like protein with similarity to a family of eukaryotic proteins that includes Ctf4p from Saccharomyces cerevisiae, sepB from Aspergillus nidulans, and AND-1 from ... [more]

Eukaryotic Cell Oct. 01, 2002; 1(5);758-73 [Pubmed: 12455694]

Throughput

Low Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: heat sensitivity (APO:0000147)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
POL1 MCL1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Tsutsui Y (2005)	Low	-	PomBase	-
MCL1 POL1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Tsutsui Y (2005)	Low	-	PomBase	-
MCL1 POL1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Williams DR (2005)	Low	-	BioGRID	-
MCL1 POL1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Williams DR (2005)	Low	-	BioGRID	-
POL1 MCL1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tsutsui Y (2005)	Low	-	BioGRID	248186
POL1 MCL1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Tsutsui Y (2005)	Low	-	PomBase	-
MCL1 POL1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Tsutsui Y (2005)	Low	-	PomBase	-

Curated By

BioGRID

Interaction Summary

MCL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

POL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA replication origin binding [IDA]DNA-directed DNA polymerase activity [IDA, IGI]chromatin binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]