Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A census of human soluble protein complexes.

Havugimana PC, Hart GT, Nepusz T, Yang H, Turinsky AL, Li Z, Wang PI, Boutz DR, Fong V, Phanse S, Babu M, Craig SA, Hu P, Wan C, Vlasblom J, Dar VU, Bezginov A, Clark GW, Wu GC, Wodak SJ, Tillier ER, Paccanaro A, Marcotte EM, Emili A

Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, ... [more]

Cell Aug. 31, 2012; 150(5);1068-81 [Pubmed: 22939629]

Quantitative Score

0.876 [Denoised score]

Throughput

High Throughput

Additional Notes

Denoised score >= 0.75

Curated By

BioGRID

Interaction Summary

USP10

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IMP]ion channel binding [IDA]p53 binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin thiolesterase activity [IDA, IMP]ubiquitin-specific protease activity [IDA, IMP]

Gene Ontology Cellular Component

USP15

Gene Ontology Biological Process

Gene Ontology Molecular Function

SMAD binding [IPI]cysteine-type endopeptidase activity [IMP]identical protein binding [IPI]protein binding [IPI]transforming growth factor beta receptor binding [IPI]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA]ubiquitinated histone binding [IDA]