AMS2
Gene Ontology Biological Process
- CENP-A containing nucleosome assembly [IMP]
- cellular protein localization [IMP]
- mitotic sister chromatid segregation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein localization to chromosome, centromeric region [IMP]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- centromeric DNA binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- centromeric DNA binding [IDA]
- core promoter proximal region sequence-specific DNA binding [IMP]
Gene Ontology Cellular Component
RAF1
Gene Ontology Biological Process
- cellular protein localization [IMP]
- chromatin silencing at centromere [IMP]
- chromatin silencing at silent mating-type cassette [IMP]
- chromatin silencing at telomere [IMP]
- chromatin silencing by small RNA [IMP]
- maintenance of chromatin silencing at silent mating-type cassette [NAS]
- meiotic chromosome segregation [IMP]
- meiotic sister chromatid cohesion, centromeric [IMP]
- meiotic telomere clustering [IMP]
- mitotic sister chromatid segregation [IMP]
- negative regulation of histone H3-K4 methylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of histone H3-K9 methylation [IMP]
- regulation of mating type switching [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Hierarchical Modularity and the Evolution of Genetic Interactomes across Species.
To date, cross-species comparisons of genetic interactomes have been restricted to small or functionally related gene sets, limiting our ability to infer evolutionary trends. To facilitate a more comprehensive analysis, we constructed a genome-scale epistasis map (E-MAP) for the fission yeast Schizosaccharomyces pombe, providing phenotypic signatures for ∼60% of the nonessential genome. Using these signatures, we generated a catalog of ... [more]
Quantitative Score
- -7.339405262 [S score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) approach was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 1.8 for positive interactions (epistatic or suppressor interactions) and S score < -2.3 for negative interactions (synthetic sick/lethal interactions).
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
AMS2 RAF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID