PGR
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- receptor binding [IPI]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- enzyme binding [IPI]
- protein binding [IPI]
- receptor binding [IPI]
Gene Ontology Cellular Component
PARP1
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Association of the Ku autoantigen/DNA-dependent protein kinase holoenzyme and poly(ADP-ribose) polymerase with the DNA binding domain of progesterone receptors.
Ligand-activated progesterone receptors (PR) bind to DNA at specific progesterone response elements by means of a DNA binding domain (DBD(PR)) containing two highly conserved zinc fingers. DNA-bound PRs regulate transcription via interaction with other nuclear proteins and transcription factors. We have now identified four HeLa cell nuclear proteins that copurify with a glutathionine-S-transferase-human DBD(PR )fusion protein. Microsequence and immunoblot analyses ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID