PHB2
Gene Ontology Biological Process
- mammary gland alveolus development [IMP]
- mammary gland branching involved in thelarche [IMP]
- negative regulation of intracellular estrogen receptor signaling pathway [IGI, IMP]
- negative regulation of mammary gland epithelial cell proliferation [IMP]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- regulation of branching involved in mammary gland duct morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MYOD1
Gene Ontology Biological Process
- cardiac muscle cell differentiation [NAS]
- cellular response to estradiol stimulus [IDA]
- cellular response to glucocorticoid stimulus [IDA]
- cellular response to oxygen levels [IDA]
- cellular response to starvation [IDA]
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- muscle organ development [IDA, IGI]
- myoblast differentiation [IDA]
- myoblast fate determination [IDA]
- myoblast fusion [IMP]
- myotube cell development [ISO]
- myotube differentiation [IDA]
- myotube differentiation involved in skeletal muscle regeneration [IMP]
- negative regulation of myoblast proliferation [IMP]
- positive regulation of muscle cell differentiation [ISO]
- positive regulation of myoblast fusion [ISO]
- positive regulation of skeletal muscle tissue regeneration [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of RNA splicing [ISO]
- regulation of alternative mRNA splicing, via spliceosome [IDA, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- skeletal muscle cell differentiation [IMP, NAS]
- skeletal muscle fiber adaptation [IMP]
- skeletal muscle fiber development [IMP]
- skeletal muscle tissue development [IDA, IGI, IMP, TAS]
- striated muscle cell differentiation [IMP]
- transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, ISO]
- chromatin DNA binding [IDA]
- chromatin binding [IDA]
- core promoter binding [IDA]
- enzyme binding [IPI]
- nuclear hormone receptor binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Akt binds prohibitin 2 and relieves its repression of MyoD and muscle differentiation.
In a yeast two-hybrid screen using the full-length Akt as bait, we found that prohibitin 2 (PHB2) specifically interacts with Akt. The C terminus of Akt (amino acids 413-480) and a central region of PHB2 (amino acids 120-232) are responsible for their mutual interaction. PHB2 acts as a transcriptional repressor in cells. PHB2 interacts with both MyoD and MEF2, and ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID