BAIT

ITPR1

D6Pas2, ENSMUSG00000072853, Gm10429, IP3R1, InsP3R, Ip3r, Itpr-1, P400, Pcp-1, Pcp1, opt
inositol 1,4,5-trisphosphate receptor 1
GO Process (14)
GO Function (8)
GO Component (24)
Mus musculus
PREY

GRM1

4930455H15Rik, ENSMUSG00000075319, Gm10828, Gprc1a, mGluR1, nmf373, rcw, wobl
glutamate receptor, metabotropic 1
GO Process (15)
GO Function (5)
GO Component (14)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Signaling complex formation of phospholipase Cbeta4 with metabotropic glutamate receptor type 1alpha and 1,4,5-trisphosphate receptor at the perisynapse and endoplasmic reticulum in the mouse brain.

Nakamura M, Sato K, Fukaya M, Araishi K, Aiba A, Kano M, Watanabe M

Upon activation of cell surface receptors coupled to the Gq subclass of G proteins, phospholipase C (PLC) beta hydrolyses membrane phospholipid to yield a pair of second messengers, inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol. PLCbeta4 has been characterized as the isoform enriched in cerebellar Purkinje cells (PCs) and the retina and involved in motor and visual functions. Here we examined cellular ... [more]

Eur. J. Neurosci. Dec. 01, 2004; 20(11);2929-44 [Pubmed: 15579147]

Throughput

  • Low Throughput

Curated By

  • BioGRID