BAIT

SPAG16

4921511D23Rik, 4930524F24Rik, 4930585K05Rik, AV261009, Pf20, Wdr29
sperm associated antigen 16
GO Process (2)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

MEIG1

MLZ-278, Meg1, RP23-167G19.5
meiosis expressed gene 1
GO Process (0)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Haploinsufficiency for the murine orthologue of Chlamydomonas PF20 disrupts spermatogenesis.

Zhang Z, Kostetskii I, Moss SB, Jones BH, Ho C, Wang H, Kishida T, Gerton GL, Radice GL, Strauss JF

PF20 was first identified in Chlamydomonas rheinhardtii as an essential component of the axoneme central apparatus. We discovered that the mouse Pf20 gene encodes two major transcripts (2.5 and 1.4 kb), which are expressed in different patterns during spermatogenesis, yielding proteins of 71 and 35 kDa, respectively. Both proteins contain contiguous WD repeats in their C termini. The meiotically expressed ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 31, 2004; 101(35);12946-51 [Pubmed: 15328412]

Throughput

  • Low Throughput

Curated By

  • BioGRID