BAIT
TSC2
Rc
tuberous sclerosis 2
GO Process (34)
GO Function (7)
GO Component (18)
Gene Ontology Biological Process
- acute-phase response [IMP]
- cell projection organization [IGI, IMP]
- cellular response to insulin stimulus [ISO]
- establishment of protein localization [IMP]
- glucose import [ISO]
- heart development [ISO]
- insulin-like growth factor receptor signaling pathway [ISO]
- negative regulation of MAP kinase activity [IMP]
- negative regulation of TOR signaling [IDA, ISO]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of cell size [IMP]
- negative regulation of epithelial cell proliferation [IDA]
- negative regulation of insulin receptor signaling pathway [IBA]
- negative regulation of phosphatidylinositol 3-kinase signaling [ISO]
- negative regulation of protein kinase B signaling [IBA, ISO]
- negative regulation of protein kinase activity [ISO]
- neural tube closure [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive chemotaxis [ISO]
- positive regulation of Rab GTPase activity [TAS]
- positive regulation of Ras GTPase activity [IBA]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein heterooligomerization [IDA, IMP]
- protein homooligomerization [IDA, IMP]
- protein import into nucleus [ISO]
- protein kinase B signaling [ISO]
- protein localization [ISO]
- protein transport into plasma membrane raft [ISO]
- regulation of cell cycle [IMP]
- regulation of endocytosis [ISO]
- regulation of insulin receptor signaling pathway [ISO]
- response to hypoxia [ISO]
- signal transduction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- TSC1-TSC2 complex [IBA, ISO]
- caveola [IDA]
- cell [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [IDA, ISO]
- dendrite [IDA]
- growth cone [IDA]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA]
- membrane [ISO]
- membrane raft [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [IDA, ISO]
- protein complex [IDA]
Rattus norvegicus
PREY
ESR1
ER-alpha, Esr, RNESTROR
estrogen receptor 1
GO Process (46)
GO Function (19)
GO Component (11)
Gene Ontology Biological Process
- Sertoli cell development [IEP]
- Sertoli cell proliferation [IMP]
- androgen metabolic process [ISO]
- antral ovarian follicle growth [ISO]
- baculum development [IEP]
- cellular response to estradiol stimulus [IDA]
- cellular response to estrogen stimulus [ISO]
- decidualization [IEP]
- epithelial cell development [ISO]
- epithelial cell proliferation involved in mammary gland duct elongation [ISO]
- intracellular receptor signaling pathway [ISO]
- intracellular steroid hormone receptor signaling pathway [IDA]
- male gonad development [IEP, ISO]
- mammary gland alveolus development [ISO]
- mammary gland branching involved in pregnancy [ISO]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- negative regulation of gene expression [ISO]
- negative regulation of glucose import [IDA]
- negative regulation of neuron death [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO, ISS]
- negative regulation of smooth muscle cell proliferation [IMP]
- negative regulation of triglyceride metabolic process [IDA]
- osteoblast development [IEP]
- phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of epidermal growth factor receptor signaling pathway [IMP]
- positive regulation of fibroblast proliferation [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO, ISS]
- positive regulation of nitric-oxide synthase activity [ISO, ISS]
- positive regulation of phospholipase C activity [IDA]
- positive regulation of retinoic acid receptor signaling pathway [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO, ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISO, TAS]
- prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis [ISO]
- prostate epithelial cord elongation [ISO]
- regulation of apoptotic process [ISO]
- regulation of branching involved in prostate gland morphogenesis [ISO]
- regulation of neuron apoptotic process [IDA]
- regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to estradiol [IEP, ISO]
- response to estrogen [ISO]
- transcription from RNA polymerase II promoter [ISO]
- uterus development [ISO]
- vagina development [ISO]
Gene Ontology Molecular Function- DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [ISO]
- chromatin binding [ISO, ISS]
- core promoter sequence-specific DNA binding [IDA, ISO]
- enzyme binding [IPI, ISO]
- estrogen receptor activity [IDA, TAS]
- estrogen response element binding [ISO]
- estrogen-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- hormone binding [IDA, IMP]
- identical protein binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- steroid binding [IDA]
- steroid hormone receptor activity [IDA]
- transcription factor binding [ISO]
- type 1 metabotropic glutamate receptor binding [IPI]
- DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- beta-catenin binding [ISO]
- chromatin binding [ISO, ISS]
- core promoter sequence-specific DNA binding [IDA, ISO]
- enzyme binding [IPI, ISO]
- estrogen receptor activity [IDA, TAS]
- estrogen response element binding [ISO]
- estrogen-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- hormone binding [IDA, IMP]
- identical protein binding [ISO]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- steroid binding [IDA]
- steroid hormone receptor activity [IDA]
- transcription factor binding [ISO]
- type 1 metabotropic glutamate receptor binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Estrogen-induced smooth muscle cell growth is regulated by tuberin and associated with altered activation of platelet-derived growth factor receptor-beta and ERK-1/2.
The mechanisms that regulate the diverse responses to estrogen (E2) are unknown. Loss of function of the tuberous sclerosis 2 gene (TSC2), a tumor suppressor gene, has been associated with a growth-promoting effect of E2. We hypothesized that tuberin, the protein product of TSC2, binds to estrogen receptors (ER) and regulates the growth effect of E2. An in vivo association ... [more]
J. Biol. Chem. May. 28, 2004; 279(22);23114-22 [Pubmed: 15039427]
Throughput
- Low Throughput
Curated By
- BioGRID