BAIT

CDPK6

CALCIUM-DEPENDENT PROTEIN KINASE 6, CPK3, Calcium dependent protein kinase 3, F9D16.120, F9D16_120, AT4G23650
calcium-dependent protein kinase 6
Arabidopsis thaliana (Columbia)
PREY

ATKCO1

ATTPK1, CA2+ ACTIVATED OUTWARD RECTIFYING K+ CHANNEL 1, KCO1, MDF20.7, MDF20_7, TANDEM PORE K+ CHANNEL 1, TPK1, TWO PORE K CHANNEL, TWO PORE K CHANNEL 1, AT5G55630
two-pore potassium channel 1
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Salt stress triggers phosphorylation of the Arabidopsis vacuolar K+ channel TPK1 by calcium dependent protein kinases (CDPKs).

Latz A, Mehlmer N, Zapf S, Mueller TD, Wurzinger B, Pfister B, Csaszar E, Hedrich R, Teige M, Becker D

14-3-3 proteins play an important role in the regulation of many cellular processes. The Arabidopsis vacuolar two pore K+ channel 1 (TPK1) interacts with the 14-3-3 protein GRF6 (GF14-λ). Upon phosphorylation of the putative binding motif in the N-terminus of TPK1, GRF6 binds to TPK1 and activates the potassium channel. In order to gain a deeper understanding of this 14-3-3 ... [more]

Mol Plant Dec. 19, 2012; 0(0); [Pubmed: 23253603]

Throughput

  • Low Throughput

Curated By

  • BioGRID