An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Molecular characterization of the ankle-link complex in cochlear hair cells and its role in the hair bundle functioning.

Michalski N, Michel V, Bahloul A, Lefevre G, Barral J, Yagi H, Chardenoux S, Weil D, Martin P, Hardelin JP, Sato M, Petit C

Several lines of evidence indicate that very large G-protein-coupled receptor 1 (Vlgr1) makes up the ankle links that connect the stereocilia of hair cells at their base. Here, we show that the transmembrane protein usherin, the putative transmembrane protein vezatin, and the PDZ (postsynaptic density-95/Discs large/zona occludens-1) domain-containing submembrane protein whirlin are colocalized with Vlgr1 at the stereocilia base in ... [more]

J. Neurosci. Jun. 13, 2007; 27(24);6478-88 [Pubmed: 17567809]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

USH2A

Gene Ontology Biological Process

Gene Ontology Molecular Function

collagen binding [IDA, ISO]myosin binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

VEZT