APH1A
Gene Ontology Biological Process
- Notch receptor processing [IMP, TAS]
- Notch signaling pathway [TAS]
- amyloid precursor protein catabolic process [IMP, TAS]
- apoptotic signaling pathway [TAS]
- membrane protein ectodomain proteolysis [IDA]
- membrane protein intracellular domain proteolysis [IMP, TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of catalytic activity [IDA]
- protein processing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PSEN1
Gene Ontology Biological Process
- Notch receptor processing [IBA, TAS]
- amyloid precursor protein catabolic process [IBA, TAS]
- beta-amyloid metabolic process [IBA]
- calcium ion transmembrane transport [IMP]
- canonical Wnt signaling pathway [IBA]
- endoplasmic reticulum calcium ion homeostasis [IDA, IGI]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- membrane protein ectodomain proteolysis [IDA]
- negative regulation of apoptotic process [IDA]
- positive regulation of catalytic activity [IDA]
- protein processing [IDA]
- regulation of phosphorylation [IDA]
- single organismal cell-cell adhesion [IMP]
- smooth endoplasmic reticulum calcium ion homeostasis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IBA]
- cell cortex [IBA]
- cell surface [IBA]
- centrosome [IDA]
- ciliary rootlet [IBA]
- dendritic shaft [IBA]
- endoplasmic reticulum [IDA]
- gamma-secretase complex [IDA]
- growth cone [IBA]
- integral component of membrane [TAS]
- integral component of plasma membrane [IDA]
- kinetochore [IDA]
- lysosomal membrane [IBA]
- membrane [IDA]
- membrane raft [IBA, IDA]
- mitochondrial inner membrane [IBA]
- mitochondrion [IDA]
- neuromuscular junction [IBA]
- neuronal cell body [IBA]
- nuclear membrane [IDA]
- nuclear outer membrane [IDA]
- perinuclear region of cytoplasm [IBA]
- rough endoplasmic reticulum [IDA]
- smooth endoplasmic reticulum [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Coordinated and widespread expression of gamma-secretase in vivo: evidence for size and molecular heterogeneity.
Gamma-secretase is a high molecular weight protein complex composed of four subunits, namely, presenilin (PS; 1 or 2), nicastrin, anterior pharynx defective-1 (Aph-1; A or B), and presenilin enhancer-2 (Pen-2), and is responsible for the cleavage of a number of type-1 transmembrane proteins. A fundamental question is whether different gamma-secretase complexes exist in vivo. We demonstrate here by in situ ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PSEN1 APH1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
PSEN1 APH1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
PSEN1 APH1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
APH1A PSEN1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - |
Curated By
- BioGRID