BAIT

EGR2

Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6
early growth response 2
GO Process (23)
GO Function (10)
GO Component (2)

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

PIASxbeta acts as an activator of Hoxb1 and is antagonized by Krox20 during hindbrain segmentation.

Garcia-Dominguez M, Gilardi-Hebenstreit P, Charnay P

The zinc-finger transcription factor Krox20 constitutes a key regulator of hindbrain development, essential for the formation and specification of rhombomeres (r) 3 and 5. It is in particular responsible for the respective activation and repression of odd- and even-numbered rhombomere-specific genes, which include Hox genes. In this study, we have identified PIASxbeta as a novel direct interactor of Krox20. In ... [more]

EMBO J. Jun. 07, 2006; 25(11);2432-42 [Pubmed: 16675951]

Throughput

  • Low Throughput

Curated By

  • BioGRID