An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1.

Heix J, Zomerdijk JC, Ravanpay A, Tjian R, Grummt I

Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein complexes containing TATA box binding protein (TBP) and specific TBP-associated factors (TAFs). Unlike class II- and III-specific TBP-TAF complexes, the corresponding murine and human class I-specific transcription initiation factor TIF-IB/SL1 exhibits a pronounced selectivity for its homologous promoter. As a first step toward understanding ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 04, 1997; 94(5);1733-8 [Pubmed: 9050847]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TAF1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase I CORE element sequence-specific DNA binding [ISO]RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity [ISO]protein binding [IPI]

Gene Ontology Cellular Component

TAF1C