BAIT
HDAC6
Hd6, Hdac5, Sfc6, mHDA2, RP23-198C2.1
histone deacetylase 6
GO Process (34)
GO Function (15)
GO Component (19)
Gene Ontology Biological Process
- Hsp90 deacetylation [IMP, ISO]
- aggresome assembly [IGI, ISO]
- cellular response to hydrogen peroxide [ISO]
- cellular response to misfolded protein [IMP]
- cellular response to topologically incorrect protein [ISO]
- histone deacetylation [IDA, ISO]
- intracellular protein transport [ISO]
- lysosome localization [ISO]
- macroautophagy [ISO]
- misfolded or incompletely synthesized protein catabolic process [ISO]
- negative regulation of microtubule depolymerization [IDA]
- negative regulation of protein complex disassembly [ISO]
- negative regulation of proteolysis [ISO]
- peptidyl-lysine deacetylation [ISO]
- polyubiquitinated misfolded protein transport [ISO]
- positive regulation of chaperone-mediated protein complex assembly [ISO]
- positive regulation of epithelial cell migration [ISO]
- positive regulation of hydrogen peroxide-mediated programmed cell death [ISO]
- positive regulation of receptor biosynthetic process [ISO]
- positive regulation of signal transduction [ISO]
- protein complex disassembly [IGI]
- protein deacetylation [IDA, ISO]
- protein polyubiquitination [IDA]
- regulation of establishment of protein localization [IMP]
- regulation of fat cell differentiation [IMP]
- regulation of gene expression, epigenetic [ISO]
- regulation of receptor activity [ISO]
- response to growth factor [ISO]
- response to misfolded protein [ISO]
- response to organic substance [ISO]
- response to toxic substance [ISO]
- tubulin deacetylation [IDA, IMP, ISO]
- ubiquitin-dependent protein catabolic process [IGI, IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function- Hsp90 protein binding [ISO]
- alpha-tubulin binding [ISO]
- beta-catenin binding [ISO]
- beta-tubulin binding [IDA]
- core promoter binding [ISO]
- dynein complex binding [ISO]
- histone deacetylase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- microtubule binding [IDA, ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- tau protein binding [ISO]
- tubulin deacetylase activity [IDA, ISO]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [ISO]
- Hsp90 protein binding [ISO]
- alpha-tubulin binding [ISO]
- beta-catenin binding [ISO]
- beta-tubulin binding [IDA]
- core promoter binding [ISO]
- dynein complex binding [ISO]
- histone deacetylase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- microtubule binding [IDA, ISO]
- polyubiquitin binding [ISO]
- protein binding [IPI]
- tau protein binding [ISO]
- tubulin deacetylase activity [IDA, ISO]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- aggresome [ISO]
- axon [IDA, ISO]
- caveola [ISO]
- cell leading edge [ISO]
- centrosome [ISO]
- cytoplasm [IDA]
- cytoplasmic microtubule [IDA]
- cytosol [IDA]
- dendrite [IDA]
- dynein complex [ISO]
- histone deacetylase complex [ISO]
- inclusion body [ISO]
- microtubule [ISO]
- microtubule associated complex [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, TAS]
- perikaryon [IDA]
- perinuclear region of cytoplasm [ISO]
- protein complex [IPI]
Mus musculus
PREY
VCP
3110001E05, CDC48, p97, p97/VCP, RP23-124L1.5
valosin containing protein
GO Process (19)
GO Function (13)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ER to Golgi vesicle-mediated transport [ISO]
- ER-associated ubiquitin-dependent protein catabolic process [ISO]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- aggresome assembly [IGI]
- cellular response to DNA damage stimulus [ISO]
- double-strand break repair [ISO]
- positive regulation of Lys63-specific deubiquitinase activity [ISO]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein K63-linked deubiquitination [ISO]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein N-linked glycosylation via asparagine [ISO]
- protein hexamerization [ISO]
- protein homooligomerization [ISO]
- protein ubiquitination [ISO]
- retrograde protein transport, ER to cytosol [ISO]
- translesion synthesis [ISO]
- ubiquitin-dependent protein catabolic process [IGI]
Gene Ontology Molecular Function- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- deubiquitinase activator activity [ISO]
- identical protein binding [ISO]
- poly(A) RNA binding [ISO]
- polyubiquitin binding [IDA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- myelin sheath [IDA]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- proteasome complex [ISO]
- protein complex [IPI]
- site of double-strand break [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
HDAC6-p97/VCP controlled polyubiquitin chain turnover.
HDAC6 is a unique cytoplasmic deacetylase capable of interacting with ubiquitin. Using a combination of biophysical, biochemical and biological approaches, we have characterized the ubiquitin-binding domain of HDAC6, named ZnF-UBP, and investigated its biological functions. These studies show that the three Zn ion-containing HDAC6 ZnF-UBP domain presents the highest known affinity for ubiquitin monomers and mediates the ability of HDAC6 ... [more]
EMBO J. Jul. 26, 2006; 25(14);3357-66 [Pubmed: 16810319]
Throughput
- Low Throughput
Curated By
- BioGRID