A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Phosphatidylinositol-4-phosphate 5-kinase regulates fission yeast cell integrity through a phospholipase C-mediated protein kinase C-independent pathway.

Deng L, Sugiura R, Ohta K, Tada K, Suzuki M, Hirata M, Nakamura S, Shuntoh H, Kuno T

Fission yeast its3-1 mutant is an allele of the essential gene its3+ that encodes a phosphatidylinositol-4-phosphate 5-kinase (PIP5K) that produces phosphatidylinositol 4,5-bisphosphate. We found that the its3-1 mutant is sensitive to micafungin, a (1,3)-beta-D-glucan synthase inhibitor, suggesting a cell wall integrity defect. Consistently, its3-1 mutation caused synthetic lethality with a (1,3)-beta-D-glucan synthase mutant, bgs1-i2, and its3-1 mutant cells showed aberrant ... [more]

J. Biol. Chem. Jul. 29, 2005; 280(30);27561-8 [Pubmed: 15923187]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Additional Notes

overexpression of its3 is lethal in the presence of the bgs1-i2 mutant

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BGS1 ITS3	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Deng L (2005)	Low	-	BioGRID	-
ITS3 BGS1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Deng L (2005)	Low	-	BioGRID	248181

Curated By

BioGRID

Interaction Summary

BGS1

Gene Ontology Biological Process

Gene Ontology Cellular Component

ITS3