PCP2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GNAO1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP, ISO, TAS]
- GTP catabolic process [ISO]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IBA]
- aging [ISO]
- cellular process [IMP]
- dopamine receptor signaling pathway [IMP]
- locomotory behavior [IMP]
- negative regulation of calcium ion transport [ISO]
- positive regulation of GTPase activity [ISO]
- regulation of heart contraction [IMP]
- response to organonitrogen compound [ISO]
Gene Ontology Molecular Function- G-protein beta/gamma-subunit complex binding [IBA]
- G-protein coupled serotonin receptor binding [IBA, ISO]
- GTP binding [IMP, ISO]
- GTPase activating protein binding [ISO]
- GTPase activity [IDA, ISO, TAS]
- corticotropin-releasing hormone receptor 1 binding [IBA, ISO]
- mu-type opioid receptor binding [IBA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- signal transducer activity [IBA]
- G-protein beta/gamma-subunit complex binding [IBA]
- G-protein coupled serotonin receptor binding [IBA, ISO]
- GTP binding [IMP, ISO]
- GTPase activating protein binding [ISO]
- GTPase activity [IDA, ISO, TAS]
- corticotropin-releasing hormone receptor 1 binding [IBA, ISO]
- mu-type opioid receptor binding [IBA, ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- signal transducer activity [IBA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. Evidence for a novel nucleotide exchange factor.
The heterotrimeric G protein Galphao is ubiquitously expressed throughout the central nervous system, but many of its functions remain to be defined. To search for novel proteins that interact with Galphao, a mouse brain library was screened using the yeast two-hybrid interaction system. Pcp2 (Purkinje cell protein-2) was identified as a partner for Galphao in this system. Pcp2 is expressed ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GNAO1 PCP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID