BAIT

DUO1

L000004421, YGL061C
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
Saccharomyces cerevisiae (S288c)
PREY

MAD1

coiled-coil domain-containing protein MAD1, L000000974, YGL086W
Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Suppressors of ipl1-2 in Components of a Glc7 Phosphatase Complex, Cdc48 AAA ATPase, TORC1, and the Kinetochore.

Robinson LC, Phillips J, Brou L, Boswell EP, Tatchell K

Ipl1/Aurora B is the catalytic subunit of a protein kinase complex required for chromosome segregation and nuclear division. Before anaphase, Ipl1 is required to establish proper kinetochore-microtubule associations and to regulate the spindle assembly checkpoint (SAC). The phosphatase Glc7/PP1 opposes Ipl1 for these activities. To investigate Ipl1 and Glc7 regulation in more detail, we isolated and characterized mutations in the ... [more]

G3 (Bethesda) Dec. 01, 2012; 2(12);1687-701 [Pubmed: 23275890]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • duo1-S115F allele

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAD1 DUO1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High0.0337BioGRID
822452

Curated By

  • BioGRID