UPF1
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA repair [IDA]
- DNA replication [IMP]
- RNA metabolic process [TAS]
- gene expression [TAS]
- histone mRNA catabolic process [IMP]
- mRNA export from nucleus [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA, IMP, NAS, TAS]
- regulation of translational termination [IMP, NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RPS3
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IBA, IDA]
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- cellular response to DNA damage stimulus [IEP]
- cytoplasmic translation [IBA]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of DNA repair [IMP]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- positive regulation of DNA N-glycosylase activity [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of apoptotic signaling pathway [IDA]
- translation [IC, NAS, TAS]
- translational elongation [TAS]
- translational initiation [NAS, TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function- DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
- NF-kappaB binding [IPI]
- damaged DNA binding [IDA]
- enzyme binding [IPI]
- iron-sulfur cluster binding [NAS]
- mRNA binding [IDA]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase A binding [IPI]
- protein kinase binding [IPI]
- structural constituent of ribosome [IDA, NAS]
- DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
- NF-kappaB binding [IPI]
- damaged DNA binding [IDA]
- enzyme binding [IPI]
- iron-sulfur cluster binding [NAS]
- mRNA binding [IDA]
- oxidized purine nucleobase lesion DNA N-glycosylase activity [IBA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase A binding [IPI]
- protein kinase binding [IPI]
- structural constituent of ribosome [IDA, NAS]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades mRNAs containing premature translation termination codons (PTCs). SMG-1 and Upf1 transiently form a surveillance complex termed "SURF" that includes eRF1 and eRF3 on post-spliced mRNAs during recognition of PTC. If an exon junction complex (EJC) exists downstream from the SURF complex, SMG-1 phosphorylates Upf1, the step that is ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 3.
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RPS3 UPF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.5409 | BioGRID | 3234092 |
Curated By
- BioGRID