BAIT

GATA6

AA410133, GATA-6
GATA binding protein 6
GO Process (41)
GO Function (14)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Mus musculus
PREY

TTF1

AV245725, TTF-1, TTF-I, RP23-426D2.3
transcription termination factor, RNA polymerase I
GO Process (3)
GO Function (3)
GO Component (1)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

GATA-6 and thyroid transcription factor-1 directly interact and regulate surfactant protein-C gene expression.

Liu C, Glasser SW, Wan H, Whitsett JA

GATA-6, a member of the GATA family of zinc finger domain containing transcription factors, is expressed in endodermally derived tissues including the lung, where GATA-6 influences the transcription of target genes, TTF-1, and surfactant proteins. Whereas GATA-6 did not directly alter expression of sp-C constructs in HeLa cells, GATA-6 synergistically activated sp-C gene transcription when co-expressed with TTF-1, supporting the ... [more]

J. Biol. Chem. Feb. 08, 2002; 277(6);4519-25 [Pubmed: 11733512]

Throughput

  • Low Throughput

Curated By

  • BioGRID