BAIT
CHRDL2
1810022C01Rik, Chl2
chordin-like 2
GO Process (2)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
BMP2
AI467020, Bmp2a, RP23-384M14.3
bone morphogenetic protein 2
GO Process (86)
GO Function (12)
GO Component (5)
Gene Ontology Biological Process
- BMP signaling pathway [IDA, IGI, ISO]
- BMP signaling pathway involved in heart induction [ISO]
- Notch signaling pathway [IMP]
- SMAD protein signal transduction [IBA, ISO]
- activation of MAPK activity [ISO]
- atrioventricular valve morphogenesis [IMP]
- bone mineralization [IDA, IGI]
- bone mineralization involved in bone maturation [ISO]
- branching involved in ureteric bud morphogenesis [IGI]
- cardiac epithelial to mesenchymal transition [ISO]
- cardiac muscle cell differentiation [ISO]
- cardiac muscle tissue morphogenesis [IMP]
- cardiocyte differentiation [ISO]
- cell fate commitment [IMP]
- cellular response to BMP stimulus [ISO]
- cellular response to growth factor stimulus [IDA]
- cellular response to organic cyclic compound [IDA]
- chondrocyte differentiation [ISO]
- corticotropin hormone secreting cell differentiation [IDA]
- embryo development [IMP]
- embryonic heart tube anterior/posterior pattern specification [IMP]
- endocardial cushion morphogenesis [IMP]
- epithelial to mesenchymal transition [ISO]
- heart development [IMP]
- in utero embryonic development [IMP]
- inner ear development [IDA]
- mesenchymal cell differentiation [ISO]
- mesenchymal cell proliferation involved in ureteric bud development [IMP]
- mesenchyme development [ISO]
- negative regulation of Wnt signaling pathway involved in heart development [ISO]
- negative regulation of aldosterone biosynthetic process [ISO]
- negative regulation of calcium-independent cell-cell adhesion [ISO]
- negative regulation of cardiac muscle cell differentiation [ISO]
- negative regulation of cell cycle [ISO]
- negative regulation of cell proliferation [IGI, ISO]
- negative regulation of cortisol biosynthetic process [ISO]
- negative regulation of gene expression [IDA]
- negative regulation of insulin-like growth factor receptor signaling pathway [ISO]
- negative regulation of steroid biosynthetic process [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISO]
- negative regulation of transcription, DNA-templated [ISO]
- odontogenesis of dentin-containing tooth [IDA]
- organ morphogenesis [IMP]
- osteoblast differentiation [IDA, IGI, ISO]
- oxidation-reduction process [IDA]
- pathway-restricted SMAD protein phosphorylation [ISO]
- pericardium development [IMP]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of Wnt signaling pathway [IDA]
- positive regulation of Wnt signaling pathway by BMP signaling pathway [IDA]
- positive regulation of apoptotic process [ISO]
- positive regulation of astrocyte differentiation [IDA, IGI]
- positive regulation of bone mineralization [ISO]
- positive regulation of cartilage development [IDA, ISO]
- positive regulation of cell differentiation [IDA]
- positive regulation of cell migration [IMP]
- positive regulation of endothelial cell proliferation [ISO]
- positive regulation of epithelial to mesenchymal transition [IMP, ISO]
- positive regulation of fat cell differentiation [IDA]
- positive regulation of gene expression [IDA, ISO]
- positive regulation of neurogenesis [ISO]
- positive regulation of neuron differentiation [IDA]
- positive regulation of odontogenesis [IDA]
- positive regulation of ossification [IDA, ISO]
- positive regulation of osteoblast differentiation [IDA, IMP, ISO]
- positive regulation of osteoblast proliferation [IMP]
- positive regulation of p38MAPK cascade [ISO]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IBA, ISO]
- positive regulation of phosphatase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- protein destabilization [IDA, IGI]
- protein phosphorylation [ISO]
- proteoglycan metabolic process [IGI]
- regulation of MAPK cascade [IBA]
- regulation of apoptotic process [IBA]
- regulation of odontogenesis of dentin-containing tooth [IGI]
- regulation of transcription, DNA-templated [ISO]
- response to hypoxia [IMP]
- telencephalon development [ISO]
- telencephalon regionalization [IGI]
- thyroid-stimulating hormone-secreting cell differentiation [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function- BMP receptor binding [IBA, ISO]
- SMAD binding [ISO]
- cytokine activity [TAS]
- growth factor activity [ISO]
- phosphatase activator activity [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- receptor binding [ISO]
- retinol dehydrogenase activity [IDA]
- transforming growth factor beta receptor binding [IBA]
- BMP receptor binding [IBA, ISO]
- SMAD binding [ISO]
- cytokine activity [TAS]
- growth factor activity [ISO]
- phosphatase activator activity [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- receptor binding [ISO]
- retinol dehydrogenase activity [IDA]
- transforming growth factor beta receptor binding [IBA]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage.
We have identified a novel chordin-like protein, CHL2, which is structurally most homologous to CHL/neuralin/ventroptin. When injected into Xenopus embryos, CHL2 RNA induced a secondary axis. Recombinant CHL2 protein interacted directly with BMPs in a competitive manner to prevent binding to the type I BMP receptor ectodomain, and inhibited BMP-dependent induction of alkaline phosphatase in C2C12 cells. Thus, CHL2 behaves ... [more]
Development Jan. 01, 2004; 131(1);229-40 [Pubmed: 14660436]
Throughput
- Low Throughput
Curated By
- BioGRID