BAIT

STAT1

2010005J02Rik, AA408197
signal transducer and activator of transcription 1
GO Process (33)
GO Function (14)
GO Component (7)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ERK and the F-box protein betaTRCP target STAT1 for degradation.

Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A

The transcription factor STAT1 has roles in development, homeostasis, cellular differentiation, and apoptosis and has been postulated to function as a tumor suppressor. STAT1 is activated by tyrosine or serine phosphorylation in response to specific cytokines or following a variety of stress-induced stimuli. STAT1 activity is carefully regulated to prevent sustained STAT1-mediated transcription, although the molecular mechanisms involved in the ... [more]

J. Biol. Chem. Jun. 06, 2008; 283(23);16077-83 [Pubmed: 18378670]

Throughput

  • Low Throughput

Curated By

  • BioGRID