BAIT

MYOCD

BSAC2A, Srfcp, RP23-298L23.1
myocardin
GO Process (40)
GO Function (9)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Mus musculus
PREY

HIST2H2BE

AV127319, H2b-613, T25626
histone cluster 2, H2be
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modulation of smooth muscle gene expression by association of histone acetyltransferases and deacetylases with myocardin.

Cao D, Wang Z, Zhang CL, Oh J, Xing W, Li S, Richardson JA, Wang DZ, Olson EN

Differentiation of smooth muscle cells is accompanied by the transcriptional activation of an array of muscle-specific genes controlled by serum response factor (SRF). Myocardin is a cardiac and smooth muscle-specific expressed transcriptional coactivator of SRF and is sufficient and necessary for smooth muscle gene expression. Here, we show that myocardin induces the acetylation of nucleosomal histones surrounding SRF-binding sites in ... [more]

Mol. Cell. Biol. Jan. 01, 2005; 25(1);364-76 [Pubmed: 15601857]

Throughput

  • Low Throughput

Additional Notes

  • acetylated histones

Curated By

  • BioGRID