BAIT

PDIA4

ERp-72, Erp70, Erp72
protein disulfide isomerase family A, member 4
GO Process (2)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

HSP90B1

Grp94, Tra1
heat shock protein 90, beta, member 1
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Multiple molecular chaperones complex with misfolded large oligomeric glycoproteins in the endoplasmic reticulum.

Kuznetsov G, Chen LB, Nigam SK

Thyroglobulin (Tg), the major protein secreted by thyroid epithelial cells and precursor of thyroid hormones, is a large dimeric glycoprotein with multiple disulfide bonds. The folding and assembly of this complex molecule begins in the endoplasmic reticulum (ER) and is likely to involve a variety of reactions catalyzed by molecular chaperones (Kuznetsov, G., Chen, L. B., and Nigam, S. K. ... [more]

J. Biol. Chem. Jan. 31, 1997; 272(5);3057-63 [Pubmed: 9006956]

Throughput

  • Low Throughput

Curated By

  • BioGRID