BAIT

HDA1

histone deacetylase HDA1, L000004088, YNL021W
Putative catalytic subunit of a class II histone deacetylase complex; role in azole resistance via Hsp90p, and in the heat shock response; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; other members of the HDA1 histone deacetylase complex are Hda2p and Hda3p
Saccharomyces cerevisiae (S288c)
PREY

MDH1

malate dehydrogenase MDH1, L000001045, YKL085W
Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated
GO Process (4)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Exploring the yeast acetylome using functional genomics.

Kaluarachchi Duffy S, Friesen H, Baryshnikova A, Lambert JP, Chong YT, Figeys D, Andrews B

Lysine acetylation is a dynamic posttranslational modification with a well-defined role in regulating histones. The impact of acetylation on other cellular functions remains relatively uncharacterized. We explored the budding yeast acetylome with a functional genomics approach, assessing the effects of gene overexpression in the absence of lysine deacetylases (KDACs). We generated a network of 463 synthetic dosage lethal (SDL) interactions ... [more]

Cell May. 11, 2012; 149(4);936-48 [Pubmed: 22579291]

Throughput

  • High Throughput|Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: colony size (APO:0000063)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • High Throughput: colony size reduction greater than 20% relative to wild type
  • Low Throughput: spot dilution assay

Curated By

  • BioGRID