An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Fbs2 is a new member of the E3 ubiquitin ligase family that recognizes sugar chains.

Yoshida Y, Tokunaga F, Chiba T, Iwai K, Tanaka K, Tai T

F-box proteins are substrate recognition components of Skp1-Cullin1-F-box protein-Roc1 (SCF) E3 ubiquitin-protein ligases. We reported previously that Fbs1 (F-box protein that recognizes sugar chains; equivalent to Fbx2 or NFB42) binds specifically to proteins attached with high mannose oligosaccharides and subsequently contributes to elimination of N-glycoproteins in cytosol (Yoshida, Y., Chiba, T., Tokunaga, F., Kawasaki, H., Iwai, K., Suzuki, T., Ito, ... [more]

J. Biol. Chem. Oct. 31, 2003; 278(44);43877-84 [Pubmed: 12939278]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

RBX1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cullin family protein binding [ISO]eukaryotic initiation factor 4E binding [TAS]protein binding [IPI]protein complex binding [ISO]ubiquitin protein ligase activity [ISO]ubiquitin-protein transferase activity [TAS]ubiquitin-ubiquitin ligase activity [ISO]

Gene Ontology Cellular Component

FETUB