PID1
Gene Ontology Biological Process
- cellular response to cytokine stimulus [ISS]
- cellular response to fatty acid [ISS]
- cellular response to interleukin-6 [ISS]
- cellular response to tumor necrosis factor [ISS, NAS]
- energy reserve metabolic process [IC]
- fat cell differentiation [IDA]
- mitochondrion morphogenesis [IMP]
- negative regulation of ATP biosynthetic process [IMP]
- negative regulation of establishment of protein localization to plasma membrane [IDA]
- negative regulation of glucose import in response to insulin stimulus [IDA]
- negative regulation of insulin receptor signaling pathway [IDA]
- negative regulation of mitochondrial DNA replication [IMP]
- negative regulation of protein phosphorylation [IDA]
- positive regulation of ATP biosynthetic process [ISS]
- positive regulation of fat cell proliferation [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of reactive oxygen species metabolic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of G1/S transition of mitotic cell cycle [IDA]
- regulation of mitochondrial fusion [IC]
- regulation of mitochondrial membrane potential [ISS, TAS]
- regulation of reactive oxygen species metabolic process [ISS]
Gene Ontology Cellular Component
LRP1
Gene Ontology Biological Process
- aorta morphogenesis [IMP]
- apoptotic cell clearance [IMP]
- beta-amyloid clearance [IMP]
- cell proliferation [ISO]
- cholesterol metabolic process [IMP]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of platelet-derived growth factor receptor-beta signaling pathway [IMP]
- negative regulation of smooth muscle cell migration [IMP]
- positive regulation of cholesterol efflux [IMP]
- positive regulation of lipid transport [IMP]
- positive regulation of protein transport [ISO]
- protein kinase C-activating G-protein coupled receptor signaling pathway [ISO]
- receptor-mediated endocytosis [IDA, ISO]
- regulation of actin cytoskeleton organization [IMP]
- regulation of cholesterol transport [IMP]
- regulation of phospholipase A2 activity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of the ligands of protein interaction domains through a functional approach.
The identification of protein-protein interaction networks has often given important information about the functions of specific proteins and on the cross-talk among metabolic and regulatory pathways. The availability of entire genome sequences has rendered feasible the systematic screening of collections of proteins, often of unknown function, aimed to find the cognate ligands. Once identified by genetic and/or biochemical approaches, the ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 6.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PID1 LRP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 800034 |
Curated By
- BioGRID