BAIT

DML1

F1O11.12, F1O11_12, REPRESSOR OF SILENCING1, ROS1, demeter-like 1, AT2G36490
protein ROS1
GO Process (5)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

ATXRCC1

T21F11.25, T21F11_25, AT1G80420
DNA-repair protein XRCC1
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The DNA repair protein XRCC1 functions in the plant DNA demethylation pathway by stimulating 5-meC excision, gap tailoring and DNA ligation.

Martinez-Macias MI, Cordoba-Canero D, Ariza RR, Roldan-Arjona T

DNA methylation patterns are the dynamic outcome of antagonist methylation and demethylation mechanisms, but the latter are still poorly understood. Active DNA demethylation in plants is mediated by a family of DNA glycosylases typified by Arabidopsis ROS1 (REPRESSOR OF SILENCING 1). ROS1 and its homologs remove 5-methylcytosine and incise the sugar backbone at the abasic site, thus initiating a base ... [more]

J. Biol. Chem. Jan. 11, 2013; 0(0); [Pubmed: 23316050]

Throughput

  • Low Throughput

Curated By

  • BioGRID