BAIT
YKU70
HDF1, NES24, KU70, L000000759, YMR284W
Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
GO Process (8)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- chromatin assembly or disassembly [IDA]
- chromatin silencing [IDA, IMP]
- chromatin silencing at silent mating-type cassette [IGI, IMP]
- double-strand break repair via break-induced replication [IGI, IMP]
- double-strand break repair via homologous recombination [IMP]
- double-strand break repair via nonhomologous end joining [IMP]
- mitochondrial double-strand break repair via homologous recombination [IMP]
- telomere maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
VPS9
VPL31, VPT9, guanine nucleotide exchange factor VPS9, L000002659, YML097C
Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a VPS9 domain; binds ubiquitin
GO Process (5)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects.
To better understand telomere biology in budding yeast, we have performed systematic suppressor/enhancer analyses on yeast strains containing a point mutation in the essential telomere capping gene CDC13 (cdc13-1) or containing a null mutation in the DNA damage response and telomere capping gene YKU70 (yku70Δ). We performed Quantitative Fitness Analysis (QFA) on thousands of yeast strains containing mutations affecting telomere-capping ... [more]
PLoS Genet. Apr. 01, 2011; 7(4);e1001362 [Pubmed: 21490951]
Quantitative Score
- -0.6283 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: heat sensitivity (APO:0000147)
Additional Notes
- yku70 null mutant; double mutants show a growth defect at 37.5 degrees; score cut-off value <= -0.5
Curated By
- BioGRID